Time dependent loss of tissue GABA content and immunoreactivity in hippocampal slices

András Mihály, Sándor L. Erdö, Ulrich Kuhnt

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Immunohistochemical detection of GABA was used to evaluate changes of the GABA innervation in hippocampal slices maintained in vitro. In parallel experiments the amount of GABA, glutamate and aspartate was measured with high performance liquid chromatography. The results showed that while glutamate and aspartate levels remained fairly constant, GABAergic neurons suffered remarkable alterations. During 8 hours' incubation the GABA content of the tissue and the number of GABA containing neuronal cell bodies decreased by 79.7% and 84.6%, respectively. The qualitative features of the immunoreactivity of the neuropil did not change. In conclusion, while in hippocampal slices tissue glutamate and aspartate levels are only slightly affected by the in vitro maintenance, more than half of the tissue GABA content is lost during prolonged in vitro incubation. As a consequence of the GABA loss, the ratio of endogenous inhibitory and excitatory amino acid transmitters has been altered, which could influence the viability of adult hippocampal tissue in in vitro conditions.

Original languageEnglish
Pages (from-to)559-564
Number of pages6
JournalBrain Research Bulletin
Volume26
Issue number4
DOIs
Publication statusPublished - Apr 1991

Keywords

  • Aspartate
  • GABA
  • Glutamate
  • Guinea pig
  • Hippocampal slice
  • Immunohistochemistry

ASJC Scopus subject areas

  • Neuroscience(all)

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