Time course of inducible nitric oxide synthase activity following endotoxin administration in dogs

Jean Charles Preiser, Haibo Zhang, Bernard Vray, Andreas Hrabak, Jean Louis Vincent

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

An increased production of nitric oxide (NO) via the inducible isoform of NO synthase (iNOS) has been incriminated in the pathogenesis of septic shock. Since the time course of iNOS activity is not known during endotoxic shock in dogs, we measured iNOS activity, estimated by the rate of conversion of 14C-arginine to 14C-citrulline in the absence of calcium, in the heart, lung, liver, kidney, and gut at 1, 2, 3, 4, and 6 h after a bolus of Escherichia coli endotoxin (2 mg/kg, iv), in the dog. This model, including generous fluid administration, is associated with typical features of human septic shock, including low systemic vascular resistance, altered myocardial function and limited oxygen extraction capability. An increase in iNOS activity was observed at 4 h in the liver (0.24 vs 0.04 mU/mg/min) and at 6 h in the heart (0.26 vs 0.09 mU/mg/min). These findings may contribute to a better delineation of the involvement of NO in endotoxic shock, and to the evaluation of the therapeutic effects of NO inhibitors.

Original languageEnglish
Pages (from-to)208-211
Number of pages4
JournalNitric Oxide - Biology and Chemistry
Volume5
Issue number2
DOIs
Publication statusPublished - Jan 1 2001

Keywords

  • Bioassay
  • Endotoxic shock
  • Lipopolysaccharide
  • Multiple organ dysfunction
  • Septic shock

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Clinical Biochemistry
  • Cancer Research

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