Time- and concentration-dependence of the growth-promoting activity of insulin and histamine in tetrahymena. Application of the MTT-method for the determination of cell proliferation in a protozoan model

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Abstract

The unicellular ciliate Tetrahymena pyriformis was treated with different concentrations of insulin or histamine and at different time points the cell density was measured, using a tetrazolium-based semiautomated colorimetric assay (MTT). The assay was suitable to determine the rate of cell proliferation of Tetrahymena. Insulin in each concentration significantly elevated the cell count up to 3 h. After that, it was neutral or its effect was insignificant. Histamine at 10-5 M concentration diminished cell count at 3, 5, 7 and 24 h. At 10-6 concentration there was no difference and at 10-7 M concentration it enhanced cell division up to 5 h, after that there being no difference. The two hormones have cell division promoting activity for cells of higher animals and the experiments demonstrate this effect already at a unicellular level.

Original languageEnglish
Pages (from-to)289-293
Number of pages5
JournalCell Biology International
Volume21
Issue number5
DOIs
Publication statusPublished - May 1997

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Tetrahymena
Histamine
Cell Count
Cell Proliferation
Insulin
Cell Division
Tetrahymena pyriformis
Hormones
growth promoting activity

Keywords

  • Cell division
  • Evolution
  • Histamine tetrahymena
  • Insulin

ASJC Scopus subject areas

  • Cell Biology

Cite this

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abstract = "The unicellular ciliate Tetrahymena pyriformis was treated with different concentrations of insulin or histamine and at different time points the cell density was measured, using a tetrazolium-based semiautomated colorimetric assay (MTT). The assay was suitable to determine the rate of cell proliferation of Tetrahymena. Insulin in each concentration significantly elevated the cell count up to 3 h. After that, it was neutral or its effect was insignificant. Histamine at 10-5 M concentration diminished cell count at 3, 5, 7 and 24 h. At 10-6 concentration there was no difference and at 10-7 M concentration it enhanced cell division up to 5 h, after that there being no difference. The two hormones have cell division promoting activity for cells of higher animals and the experiments demonstrate this effect already at a unicellular level.",
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