Tilted pillar array fabrication by the combination of proton beam writing and soft lithography for microfluidic cell capture: Part 1 Design and feasibility

I. Rajta, R. Huszánk, Atilla T T Szabó, Gyula U L Nagy, Szabolcs Szilasi, P. Fürjes, Eszter Holczer, Zoltan Fekete, Gabor Járvás, Marton Szigeti, Laszlo Hajba, Judit Bodnár, A. Guttman

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Design, fabrication, integration, and feasibility test results of a novel microfluidic cell capture device is presented, exploiting the advantages of proton beam writing to make lithographic irradiations under multiple target tilting angles and UV lithography to easily reproduce large area structures. A cell capture device is demonstrated with a unique doubly tilted micropillar array design for cell manipulation in microfluidic applications. Tilting the pillars increased their functional surface, therefore, enhanced fluidic interaction when special bioaffinity coating was used, and improved fluid dynamic behavior regarding cell culture injection. The proposed microstructures were capable to support adequate distribution of body fluids, such as blood, spinal fluid, etc., between the inlet and outlet of the microfluidic sample reservoirs, offering advanced cell capture capability on the functionalized surfaces. The hydrodynamic characteristics of the microfluidic systems were tested with yeast cells (similar size as red blood cells) for efficient capture.

Original languageEnglish
Pages (from-to)498-503
Number of pages6
JournalElectrophoresis
Volume37
Issue number3
DOIs
Publication statusPublished - Feb 1 2016

Keywords

  • Cell capture
  • Proton beam writing
  • Soft lithography

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

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