Thyrotropin powers human mitochondria

Burkhard Poeggeler, Jana Knuever, Erzsébet Gáspár, T. Bíró, Matthias Klinger, E. Bódó, Rudolf J. Wiesner, Björn E. Wenzel, Ralf Paus

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Here we demonstrate that the neuropeptide hormone thyrotropin (TSH), which controls thyroid hormone production, exerts a major nonclassical function in mitochondrial biology. Based on transcriptional, ultrastructural, immunohistochemical, and biochemical evidence, TSH up-regulates mitochondrial biogenesis and consequently activity in organ-cultured normal human epidermis in situ. Mitochondrial activity was assessed by measuring 2 key components of the respiratory chain. The abundance of mitochondria was assessed employing 2 independent morphological techniques: counting their numbers in human epidermis by high-magnification light microscopy of skin sections immunostained for mitochondria-selective cytochrome-c-oxidase subunit 1 (MTCO1) and transmission electron microscopy (TEM). Treatment with 10 mU/ml of TSH for 6 d strongly up-regulates the number of light-microscopically visualized, MTCO1-demarcated mitochondria. On the ultrastructural level, TEM confirms that TSH indeed stimulates mitochondrial proliferation and biogenesis in the perinuclear region of human skin epidermal keratinocytes. On the transcriptional level, TSH up-regulates MTCO1 mRNA (quantitative RT-PCR) and significantly enhances complex I and IV (cytochrome-c-oxidase) activity. This study pioneers the concept that mitochondrial energy metabolism and biogenesis in a normal, prototypic human epithelial tissue underlies potent neuroendocrine controls and introduces human skin organ culture as a clinically relevant tool for further exploring this novel research frontier in the control of mitochondrial biology.

Original languageEnglish
Pages (from-to)1525-1531
Number of pages7
JournalFASEB Journal
Volume24
Issue number5
DOIs
Publication statusPublished - May 2010

Fingerprint

Mitochondria
Thyrotropin
Electron Transport Complex IV
Skin
Up-Regulation
Organelle Biogenesis
Transmission Electron Microscopy
Epidermis
Transmission electron microscopy
Light
Organ Culture Techniques
Electron Transport
Neuropeptides
Thyroid Hormones
Keratinocytes
Energy Metabolism
Optical microscopy
Microscopy
Epithelium
Hormones

Keywords

  • Aging
  • Hypothalamic-pituitary-thyroid axis
  • Mitochondrial energy metabolism
  • Oxygen utilization
  • Skin

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Genetics
  • Molecular Biology
  • Medicine(all)

Cite this

Poeggeler, B., Knuever, J., Gáspár, E., Bíró, T., Klinger, M., Bódó, E., ... Paus, R. (2010). Thyrotropin powers human mitochondria. FASEB Journal, 24(5), 1525-1531. https://doi.org/10.1096/fj.09-147728

Thyrotropin powers human mitochondria. / Poeggeler, Burkhard; Knuever, Jana; Gáspár, Erzsébet; Bíró, T.; Klinger, Matthias; Bódó, E.; Wiesner, Rudolf J.; Wenzel, Björn E.; Paus, Ralf.

In: FASEB Journal, Vol. 24, No. 5, 05.2010, p. 1525-1531.

Research output: Contribution to journalArticle

Poeggeler, B, Knuever, J, Gáspár, E, Bíró, T, Klinger, M, Bódó, E, Wiesner, RJ, Wenzel, BE & Paus, R 2010, 'Thyrotropin powers human mitochondria', FASEB Journal, vol. 24, no. 5, pp. 1525-1531. https://doi.org/10.1096/fj.09-147728
Poeggeler B, Knuever J, Gáspár E, Bíró T, Klinger M, Bódó E et al. Thyrotropin powers human mitochondria. FASEB Journal. 2010 May;24(5):1525-1531. https://doi.org/10.1096/fj.09-147728
Poeggeler, Burkhard ; Knuever, Jana ; Gáspár, Erzsébet ; Bíró, T. ; Klinger, Matthias ; Bódó, E. ; Wiesner, Rudolf J. ; Wenzel, Björn E. ; Paus, Ralf. / Thyrotropin powers human mitochondria. In: FASEB Journal. 2010 ; Vol. 24, No. 5. pp. 1525-1531.
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