Thyroid hormones directly alter human hair follicle functions: Anagen prolongation and stimulation of both hair matrix keratinocyte proliferation and hair pigmentation

Nina Van Beek, E. Bódó, Arno Kromminga, Erzsébet Gáspár, Katja Meyer, Michal A. Zmijewski, Andrzej Slominski, Björn E. Wenzel, Ralf Paus

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Context: Both insufficient and excess levels of thyroid hormones (T 3 and T4) can result in altered hair/skin structureandfunction (e.g. effluvium). However, it is still unclear whether T3 and T4 exert any direct effects on human hair follicles (HFs), and if so, how exactly human HFs respond to T3/T4 stimulation. Objective: Our objective was to asses the impact of T 3/T4 on human HF in vitro. Methods: Human anagen HFs were isolated from skin obtained from females undergoing facelift surgery. HFs from euthyroid females between 40 and 69 yr (average, 56 yr) were cultured and treated with T3/T4. Results: Studying microdissected, organ-cultured normal human scalp HFs, we show here that T4 up-regulates the proliferation of hair matrix keratinocytes, whereas their apoptosis is down-regulated by T3 and T4. T4 also prolongs the duration of the hair growth phase (anagen) in vitro, possibly due to the down-regulation of TGF-β2, the key anagen-inhibitory growth factor. Because we show here that human HFs transcribe deiodinase genes (D2 and D3), they may be capable of converting T4 to T3. Intrafollicular immunoreactivity for the recognized thyroid hormone-responsive keratins cytokeratin (CK) 6 and CK14 is significantly modulated by T3 and T4 (CK6 is enhanced, CK14 down-regulated). Both T3 and T4 also significantly stimulate intrafollicular melanin synthesis. Conclusions: Thus, we present the first evidence that human HFs are direct targets of thyroid hormones and demonstrate that T3 and/or T4 modulate multiple hair biology parameters, ranging from HF cycling to pigmentation.

Original languageEnglish
Pages (from-to)4381-4388
Number of pages8
JournalJournal of Clinical Endocrinology and Metabolism
Volume93
Issue number11
DOIs
Publication statusPublished - Nov 2008

Fingerprint

Hair Follicle
Pigmentation
Thyroid Hormones
Keratinocytes
Hair
Skin
Keratin-6
Iodide Peroxidase
Melanins
Triiodothyronine
Keratins
Thyroxine
Surgery
Genes
Apoptosis
Rhytidoplasty
Equidae
Scalp
Up-Regulation
Down-Regulation

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Endocrinology
  • Biochemistry, medical
  • Endocrinology, Diabetes and Metabolism

Cite this

Thyroid hormones directly alter human hair follicle functions : Anagen prolongation and stimulation of both hair matrix keratinocyte proliferation and hair pigmentation. / Van Beek, Nina; Bódó, E.; Kromminga, Arno; Gáspár, Erzsébet; Meyer, Katja; Zmijewski, Michal A.; Slominski, Andrzej; Wenzel, Björn E.; Paus, Ralf.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 93, No. 11, 11.2008, p. 4381-4388.

Research output: Contribution to journalArticle

Van Beek, Nina ; Bódó, E. ; Kromminga, Arno ; Gáspár, Erzsébet ; Meyer, Katja ; Zmijewski, Michal A. ; Slominski, Andrzej ; Wenzel, Björn E. ; Paus, Ralf. / Thyroid hormones directly alter human hair follicle functions : Anagen prolongation and stimulation of both hair matrix keratinocyte proliferation and hair pigmentation. In: Journal of Clinical Endocrinology and Metabolism. 2008 ; Vol. 93, No. 11. pp. 4381-4388.
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abstract = "Context: Both insufficient and excess levels of thyroid hormones (T 3 and T4) can result in altered hair/skin structureandfunction (e.g. effluvium). However, it is still unclear whether T3 and T4 exert any direct effects on human hair follicles (HFs), and if so, how exactly human HFs respond to T3/T4 stimulation. Objective: Our objective was to asses the impact of T 3/T4 on human HF in vitro. Methods: Human anagen HFs were isolated from skin obtained from females undergoing facelift surgery. HFs from euthyroid females between 40 and 69 yr (average, 56 yr) were cultured and treated with T3/T4. Results: Studying microdissected, organ-cultured normal human scalp HFs, we show here that T4 up-regulates the proliferation of hair matrix keratinocytes, whereas their apoptosis is down-regulated by T3 and T4. T4 also prolongs the duration of the hair growth phase (anagen) in vitro, possibly due to the down-regulation of TGF-β2, the key anagen-inhibitory growth factor. Because we show here that human HFs transcribe deiodinase genes (D2 and D3), they may be capable of converting T4 to T3. Intrafollicular immunoreactivity for the recognized thyroid hormone-responsive keratins cytokeratin (CK) 6 and CK14 is significantly modulated by T3 and T4 (CK6 is enhanced, CK14 down-regulated). Both T3 and T4 also significantly stimulate intrafollicular melanin synthesis. Conclusions: Thus, we present the first evidence that human HFs are direct targets of thyroid hormones and demonstrate that T3 and/or T4 modulate multiple hair biology parameters, ranging from HF cycling to pigmentation.",
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T2 - Anagen prolongation and stimulation of both hair matrix keratinocyte proliferation and hair pigmentation

AU - Van Beek, Nina

AU - Bódó, E.

AU - Kromminga, Arno

AU - Gáspár, Erzsébet

AU - Meyer, Katja

AU - Zmijewski, Michal A.

AU - Slominski, Andrzej

AU - Wenzel, Björn E.

AU - Paus, Ralf

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N2 - Context: Both insufficient and excess levels of thyroid hormones (T 3 and T4) can result in altered hair/skin structureandfunction (e.g. effluvium). However, it is still unclear whether T3 and T4 exert any direct effects on human hair follicles (HFs), and if so, how exactly human HFs respond to T3/T4 stimulation. Objective: Our objective was to asses the impact of T 3/T4 on human HF in vitro. Methods: Human anagen HFs were isolated from skin obtained from females undergoing facelift surgery. HFs from euthyroid females between 40 and 69 yr (average, 56 yr) were cultured and treated with T3/T4. Results: Studying microdissected, organ-cultured normal human scalp HFs, we show here that T4 up-regulates the proliferation of hair matrix keratinocytes, whereas their apoptosis is down-regulated by T3 and T4. T4 also prolongs the duration of the hair growth phase (anagen) in vitro, possibly due to the down-regulation of TGF-β2, the key anagen-inhibitory growth factor. Because we show here that human HFs transcribe deiodinase genes (D2 and D3), they may be capable of converting T4 to T3. Intrafollicular immunoreactivity for the recognized thyroid hormone-responsive keratins cytokeratin (CK) 6 and CK14 is significantly modulated by T3 and T4 (CK6 is enhanced, CK14 down-regulated). Both T3 and T4 also significantly stimulate intrafollicular melanin synthesis. Conclusions: Thus, we present the first evidence that human HFs are direct targets of thyroid hormones and demonstrate that T3 and/or T4 modulate multiple hair biology parameters, ranging from HF cycling to pigmentation.

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