Thrittene radioimmunoassay: Description and application of a novel method

G. Fürjes, G. Tóth, B. Peitl, R. Pórszász, B. Lelesz, R. Sári, A. Tóth, Z. Szilvássy, J. Németh

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In the present paper the development and application of a novel thrittene radioimmunoassay (RIA) are described. 125I-labeling of Tyr (0)-thrittene was performed by the iodogen-method and the mono-iodinated peptide, as RIA tracer, was separated by reversed-phase high performance liquid chromatography (HPLC). The RIA results show that the antiserum used in the radioimmunoassay turned to be C-terminal specific, without significant affinity to other members of the somatostatin peptide hormone family. Detection limit of the assay was 0.2 fmol/ml. This highly specific and sensitive thrittene RIA was used to investigate the distribution of thrittene in the rat gastrointestinal tract and other tissue samples. Different areas of the gastrointestinal tract and other tissues were removed from rats and after extraction the samples were processed for thrittene radioimmunoassay. Highest concentrations were found in the duodenum samples followed by jejunum and ileum, however, all the examined tissues contained highly enough thrittene for the measurement.

Original languageEnglish
Pages (from-to)113-118
Number of pages6
JournalJournal of Radioanalytical and Nuclear Chemistry
Volume292
Issue number1
DOIs
Publication statusPublished - Apr 2012

Fingerprint

Radioimmunoassay
Tissue
Rats
Peptide Hormones
High performance liquid chromatography
Somatostatin
Labeling
Peptides
Gastrointestinal Tract
Immune Sera
Assays
Reverse-Phase Chromatography
Jejunum
Ileum
Duodenum
Limit of Detection
High Pressure Liquid Chromatography
1,3,4,6-tetrachloro-3 alpha,6 alpha-diphenylglycoluril

Keywords

  • I
  • HPLC purification
  • Iodination
  • Radioimmunoassay
  • Thrittene

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Spectroscopy
  • Public Health, Environmental and Occupational Health
  • Pollution
  • Health, Toxicology and Mutagenesis
  • Nuclear Energy and Engineering
  • Analytical Chemistry

Cite this

Thrittene radioimmunoassay : Description and application of a novel method. / Fürjes, G.; Tóth, G.; Peitl, B.; Pórszász, R.; Lelesz, B.; Sári, R.; Tóth, A.; Szilvássy, Z.; Németh, J.

In: Journal of Radioanalytical and Nuclear Chemistry, Vol. 292, No. 1, 04.2012, p. 113-118.

Research output: Contribution to journalArticle

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