Three-Dimensional Imaging of High Performance Liquid Chromatography Processes Using a Mini Positron Emission Tomograph

Eva Sarkadi-Priboczki, Ivan Valastyan, Karoly Brezovcsik, David Nagy, Gabor Opposits, Imre Lajtos, Miklos Emri, Laszlo Balkay, Jozsef Molnar

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Positron emission tomography (PET) is a widely used technique for following radiotracer distribution, molecule specific binding or biochemical processes in living animals or in human body. Small animal PET cameras with high spatial resolution and high sensitivity are also useful, beyond their original pre-clinical application, in the following of surface chemical and the physicochemical processes. The purpose of this study was the introduction of the three-dimensional real-time PET based visualization method in separation processes. The separated tracer compounds can be followed and displayed in three dimension along chromatography column using the PET technique. We studied the separation of 11C-labeled methanol and 11C-labeled methyl iodide using reversed-phase high performance liquid chromatography (HPLC) on semi-preparative and analytical columns in order to determine the detection limits of our PET camera (MiniPET). The PET imaging is suitable for analyzing the column efficiency and the underlying processes in the liquid chromatography. Synthesis, separation and quality control of PET radiopharmaceuticals are performed in radiochemistry labs (in PET Centers) with the help of the liquid chromatography, therefore equipment, infrastructure and other lab conditions are usually available for researchers and for private companies to study chromatographic separation processes and to develop columns.

Original languageEnglish
Pages (from-to)9797-9802
Number of pages6
JournalChemistrySelect
Volume2
Issue number30
DOIs
Publication statusPublished - Jan 1 2017

Keywords

  • Compound band visualization
  • PET imaging
  • Positron emission tomography
  • Separation process follow-up
  • defective HPLC column monitoring

ASJC Scopus subject areas

  • Chemistry(all)

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