Thiolated pyrimidine derivatives may interfere thiol groups concentrated at lipid rafts of HIV-1 infected cells

Szilvia Kanizsai, Joseph Ongrádi, János Aradi, Károly Nagy

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Abstract

Upon HIV infection, cells become activated and cell surface thiols are present in increased number. Earlier we demonstrated in vitro anti-HIV effect of thiolated pyrimidine nucleotide UD29, which interferes thiol function. To further analyse the redox processes required for HIV-1 entry and infection, toxicity assays were performed using HIV-1 infected monolayer HeLaCD4-LTR/ β-gal cells and suspension H9 T cells treated with several thiolated nucleotide derivatives of UD29. Selective cytotoxicity of thiolated pyrimidines on HIV-1 infected cells were observed. Results indicate that thiolated pyrimidine derivates may interfere with -SH (thiol) groups concentrated in lipid rafts of cell membrane and interacts HIV-1 infected (activated) cells resulting in a selective cytotoxicity of HIV-1 infected cells, and reducing HIV-1 entry.

Original languageEnglish
Pages (from-to)447-458
Number of pages12
JournalActa microbiologica et immunologica Hungarica
Volume61
Issue number4
DOIs
Publication statusPublished - Dec 1 2014

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Keywords

  • Cell surface redox system
  • Entry inhibitors
  • HIV infection
  • Lipid rafts
  • Thiolated nucleotides

ASJC Scopus subject areas

  • Immunology and Microbiology(all)

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