Thin-layer chromatography-densitometry and liquid chromatography analysis of alkaloids in leaves of papaver somniferum under stress conditions

Beata Szabó, Ágnes Lakatos, Tamás Kõszegi, György Kátay, L. Botz

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The effect of stress conditions on the concentrations of secondary metabolites were examined during various developmental stages of Papaver somniferum plants. P. somniferum plants were grown in laboratory conditions (Budakalász). The experiment consisted of 22 treatments. Significantly different alkaloid contents can be observed under different stress conditions. In general, the alkaloid contents of plants are very low; therefore, a highly sensitive and reliable method has to be developed for analysis. The amount of alkaloids was measured by 2 separation and detection techniques. Accuracy of the thin-layer chromatography method for quantitative analysis is limited. Without purification of samples the background is too noisy. Column liquid chromatography is a sensitive and relatively inexpensive method that allows precise quantitative determination of the alkaloid content.

Original languageEnglish
Pages (from-to)1571-1577
Number of pages7
JournalJournal of AOAC International
Volume88
Issue number5
Publication statusPublished - Sep 1 2005

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science
  • Environmental Chemistry
  • Agronomy and Crop Science
  • Pharmacology

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