Thin-layer and high-performance liquid chromatographic determination of P388/S tumor cell and host liver polyamines

T. Kremmer, L. Holczinger, M. Boldizsár, L. Selmeci, S. Bardócz

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Thin-layer and high-performance liquid chromatographic (HPLC) methods using adsorption and reversed-phase techniques are compared for separation and quantitation of P388/S leukemia cell and host liver polyamines. An improved HPLC method on a silica gel column (Microsil, LiChrosorb Si 100) using chloroformmethanol-water as mobile phase is described and some chromatographic parameters have been studied. A reliable and reproducible resolution and determination of Dns-polyamines has been achieved on a reversed-phase (LiChrosorb RP-8) columns by means of a programmed linear methanol-water gradient with detection at 254 nm. This method is suitable for the serial analysis of polyamines in P388/S leukemia cells, in host liver and body fluids.

Original languageEnglish
Pages (from-to)371-379
Number of pages9
JournalJournal of Chromatography A
Volume286
Issue numberC
DOIs
Publication statusPublished - Mar 23 1984

Fingerprint

Polyamines
Liver
Leukemia P388
Tumors
Cells
Liquids
Neoplasms
Water
Body fluids
Silica Gel
Body Fluids
Adsorption
Methanol

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry
  • Molecular Medicine

Cite this

Thin-layer and high-performance liquid chromatographic determination of P388/S tumor cell and host liver polyamines. / Kremmer, T.; Holczinger, L.; Boldizsár, M.; Selmeci, L.; Bardócz, S.

In: Journal of Chromatography A, Vol. 286, No. C, 23.03.1984, p. 371-379.

Research output: Contribution to journalArticle

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