Thermostable phytase production by Thermoascus aurantiacus in submerged fermentation

K. Madhavan Nampoothiri, G. Jino Tomes, Krishnan Roopesh, G. Szakács, Viviana Nagy, Carlos Ricardo Soccol, Ashok Pandey

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Phytases act on phytic acid, an antinutrient factor present in animal feeds, and release inorganic phosphate. We optimized the production parameters for phytase production using Thermoascus aurantiacus (TUB F 43), a thermophilic fungal culture, by submerged fermentation. A semisynthetic medium containing glucose, starch, peptone, and minerals supplemented with 3.75% (w/v) wheat bran particles was found to be the best production medium among the various combinations tried. Further supplementation of this medium with surfactants such as Tween-20 and Tween-80 considerably enhanced the enzyme yield. A maximum phytase activity (468.22 U/mL) was obtained using this production medium containing 2% (v/v) Tween-20 after 72 h of fermentation at 45°C in shake-flask cultures with a rotation of 150 rpm. Herein we present details of a few of the process parameter optimizations. The phytase enzyme was found to be thermostable, and the optimal temperature for phytase activity was found to be 55°C. However, 80% of the activity still remained when the temperature was shifted to 70°C.

Original languageEnglish
Pages (from-to)205-214
Number of pages10
JournalApplied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
Volume118
Issue number1-3
DOIs
Publication statusPublished - Jul 2004

Fingerprint

Thermoascus
6-Phytase
Fermentation
Polysorbates
Enzymes
Starch
Peptones
Phytic Acid
Batch Cell Culture Techniques
Temperature
Glucose
Dietary Fiber
Animals
Phosphates
Surface active agents
Minerals
Surface-Active Agents
Acids

Keywords

  • Phytic acid
  • Submerged fermentation
  • Surfactants
  • Thermoascus aurantiacus
  • Thermostable phytase

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Bioengineering

Cite this

Thermostable phytase production by Thermoascus aurantiacus in submerged fermentation. / Nampoothiri, K. Madhavan; Tomes, G. Jino; Roopesh, Krishnan; Szakács, G.; Nagy, Viviana; Soccol, Carlos Ricardo; Pandey, Ashok.

In: Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology, Vol. 118, No. 1-3, 07.2004, p. 205-214.

Research output: Contribution to journalArticle

Nampoothiri, K. Madhavan ; Tomes, G. Jino ; Roopesh, Krishnan ; Szakács, G. ; Nagy, Viviana ; Soccol, Carlos Ricardo ; Pandey, Ashok. / Thermostable phytase production by Thermoascus aurantiacus in submerged fermentation. In: Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology. 2004 ; Vol. 118, No. 1-3. pp. 205-214.
@article{cf57ad81cc3f489089a4ff28f93459dd,
title = "Thermostable phytase production by Thermoascus aurantiacus in submerged fermentation",
abstract = "Phytases act on phytic acid, an antinutrient factor present in animal feeds, and release inorganic phosphate. We optimized the production parameters for phytase production using Thermoascus aurantiacus (TUB F 43), a thermophilic fungal culture, by submerged fermentation. A semisynthetic medium containing glucose, starch, peptone, and minerals supplemented with 3.75{\%} (w/v) wheat bran particles was found to be the best production medium among the various combinations tried. Further supplementation of this medium with surfactants such as Tween-20 and Tween-80 considerably enhanced the enzyme yield. A maximum phytase activity (468.22 U/mL) was obtained using this production medium containing 2{\%} (v/v) Tween-20 after 72 h of fermentation at 45°C in shake-flask cultures with a rotation of 150 rpm. Herein we present details of a few of the process parameter optimizations. The phytase enzyme was found to be thermostable, and the optimal temperature for phytase activity was found to be 55°C. However, 80{\%} of the activity still remained when the temperature was shifted to 70°C.",
keywords = "Phytic acid, Submerged fermentation, Surfactants, Thermoascus aurantiacus, Thermostable phytase",
author = "Nampoothiri, {K. Madhavan} and Tomes, {G. Jino} and Krishnan Roopesh and G. Szak{\'a}cs and Viviana Nagy and Soccol, {Carlos Ricardo} and Ashok Pandey",
year = "2004",
month = "7",
doi = "10.1385/ABAB:118:1-3:205",
language = "English",
volume = "118",
pages = "205--214",
journal = "Applied Biochemistry and Biotechnology",
issn = "0273-2289",
publisher = "Humana Press",
number = "1-3",

}

TY - JOUR

T1 - Thermostable phytase production by Thermoascus aurantiacus in submerged fermentation

AU - Nampoothiri, K. Madhavan

AU - Tomes, G. Jino

AU - Roopesh, Krishnan

AU - Szakács, G.

AU - Nagy, Viviana

AU - Soccol, Carlos Ricardo

AU - Pandey, Ashok

PY - 2004/7

Y1 - 2004/7

N2 - Phytases act on phytic acid, an antinutrient factor present in animal feeds, and release inorganic phosphate. We optimized the production parameters for phytase production using Thermoascus aurantiacus (TUB F 43), a thermophilic fungal culture, by submerged fermentation. A semisynthetic medium containing glucose, starch, peptone, and minerals supplemented with 3.75% (w/v) wheat bran particles was found to be the best production medium among the various combinations tried. Further supplementation of this medium with surfactants such as Tween-20 and Tween-80 considerably enhanced the enzyme yield. A maximum phytase activity (468.22 U/mL) was obtained using this production medium containing 2% (v/v) Tween-20 after 72 h of fermentation at 45°C in shake-flask cultures with a rotation of 150 rpm. Herein we present details of a few of the process parameter optimizations. The phytase enzyme was found to be thermostable, and the optimal temperature for phytase activity was found to be 55°C. However, 80% of the activity still remained when the temperature was shifted to 70°C.

AB - Phytases act on phytic acid, an antinutrient factor present in animal feeds, and release inorganic phosphate. We optimized the production parameters for phytase production using Thermoascus aurantiacus (TUB F 43), a thermophilic fungal culture, by submerged fermentation. A semisynthetic medium containing glucose, starch, peptone, and minerals supplemented with 3.75% (w/v) wheat bran particles was found to be the best production medium among the various combinations tried. Further supplementation of this medium with surfactants such as Tween-20 and Tween-80 considerably enhanced the enzyme yield. A maximum phytase activity (468.22 U/mL) was obtained using this production medium containing 2% (v/v) Tween-20 after 72 h of fermentation at 45°C in shake-flask cultures with a rotation of 150 rpm. Herein we present details of a few of the process parameter optimizations. The phytase enzyme was found to be thermostable, and the optimal temperature for phytase activity was found to be 55°C. However, 80% of the activity still remained when the temperature was shifted to 70°C.

KW - Phytic acid

KW - Submerged fermentation

KW - Surfactants

KW - Thermoascus aurantiacus

KW - Thermostable phytase

UR - http://www.scopus.com/inward/record.url?scp=4344684586&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4344684586&partnerID=8YFLogxK

U2 - 10.1385/ABAB:118:1-3:205

DO - 10.1385/ABAB:118:1-3:205

M3 - Article

VL - 118

SP - 205

EP - 214

JO - Applied Biochemistry and Biotechnology

JF - Applied Biochemistry and Biotechnology

SN - 0273-2289

IS - 1-3

ER -