Thermodynamic and kinetic processes during the unfolding of BSA in the presence of the mycotoxin patulin

Eszter Horváth, Nikoletta Kálmán, M. Pesti, K. Iwata, S. Kunsági-Máté

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The effects of the mycotoxin patulin on the thermodynamics and kinetics of the transition of bovine serum albumin (BSA) in aqueous solution were studied by Differential Scanning Calorimetry and Photoluminescence methods. Results show that in the presence of patulin, the free enthalpy change during the transition of BSA was decreased by an average of ∼ 46 kJ/mol, the free energy change was decreased by ∼ 4 kJ/mol, and the activation energy fell from ∼ 1546 to ∼ 840 kJ/mol. These results indicate that the bioactivity of patulin is based on the kinetic rather than on the thermodynamic properties of the transition. This is the first evidence of the direct interaction of patulin with the free thiol-containing BSA, a process which could contribute to the adverse cyto-and genotoxic effects induced by patulin.

Original languageEnglish
Pages (from-to)389-398
Number of pages10
JournalActa Biologica Hungarica
Volume63
Issue number3
DOIs
Publication statusPublished - Sep 1 2012

Fingerprint

Patulin
Mycotoxins
Bovine Serum Albumin
Thermodynamics
serum
thermodynamics
kinetics
Kinetics
bioactivity
thiol
thermodynamic property
calorimetry
enthalpy
activation energy
aqueous solution
Differential Scanning Calorimetry
Bioactivity
Sulfhydryl Compounds
Free energy
Differential scanning calorimetry

Keywords

  • activation energy
  • BSA unfolding
  • free enthalpy
  • Patulin
  • transition thermodynamics and kinetics

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Neurology

Cite this

Thermodynamic and kinetic processes during the unfolding of BSA in the presence of the mycotoxin patulin. / Horváth, Eszter; Kálmán, Nikoletta; Pesti, M.; Iwata, K.; Kunsági-Máté, S.

In: Acta Biologica Hungarica, Vol. 63, No. 3, 01.09.2012, p. 389-398.

Research output: Contribution to journalArticle

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