Thermal stability enhancement of Candida rugosa lipase using ionic liquids

O. Ulbert, K. Bélafi-Bakó, K. Tonova, L. Gubicza

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

The thermal stability of Candida rugosa (C. rugosa) lipase was investigated and compared in n-hexane, benzene, dibutylether as well as [bmim] PF6 and [omiml PF6 ionic liquids and the effect of solvent polarity and water activity were evaluated. Deactivation of the enzyme followed a series-type kinetic model. First order deactivation rate constants and the ratios of specific activities were determined and the kinetics of deactivation were studied. Among the organic solvents, the best stability was observed in n-hexane with a half-life of 6.5 h at water activity of 0.51. In ionic liquids, however, even longer half lives were obtained, and the enzyme was stable in these solvents at 50°C. The highest half-life times were obtained in [bmim] PF6 (12.3 h) and [omim] PF6 (10.6 h). A direct correlation was found between solvent polarity and thermal stability since the higher the polarity of the solvent, the lower was the stability decrease at 50°C comparing to that at 30°C.

Original languageEnglish
Pages (from-to)177-183
Number of pages7
JournalBiocatalysis and Biotransformation
Volume23
Issue number3-4
DOIs
Publication statusPublished - May 2005

Fingerprint

Ionic Liquids
Candida
Lipases
Lipase
Ionic liquids
Thermodynamic stability
Hot Temperature
Hexane
Enzymes
Half-Life
Enzyme kinetics
Kinetics
Water
Benzene
Organic solvents
Rate constants
1-butyl-3-methylimidazolium hexafluorophosphate
n-hexane

Keywords

  • Candida rugosa lipase
  • Enzyme half-life time
  • Ionic liquids
  • Thermal stability
  • Water activity

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Catalysis

Cite this

Thermal stability enhancement of Candida rugosa lipase using ionic liquids. / Ulbert, O.; Bélafi-Bakó, K.; Tonova, K.; Gubicza, L.

In: Biocatalysis and Biotransformation, Vol. 23, No. 3-4, 05.2005, p. 177-183.

Research output: Contribution to journalArticle

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