Thermal characterisation of actin filaments prepared from adp-actin monomers

J. Orbán, Kinga Pozsonyi, Krisztina Szarka, Szilvia Barkó, Emoke Bódis, D. Lőrinczy

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The thermodynamic properties of the ADP- and ATP-actin filaments were compared by the method of differential scanning calorimetry. The lower melting point for the ADP-F-actin filament (58.4 vs. 64.5°C for ATP-F-actin) indicated that compared to the ATP-actin filaments its structure was less resistant to heat denaturation. The detailed thermodynamic characterisation of the proteins was carried out by the analysis of the calorimetric enthalpy the entropy and the free enthalpy changes. All of the determined parameters gave lower values to the ADP-actin filaments than to the ATP-actin filaments. The calculated values of the activation energy also demonstrated that compared to the ADP-F-actin the ATP-F-actin was thermodynamically more resistant to the denaturing effect of heat. Based on all of this information we have concluded that the actin filament prepared from ADP containing magnesium saturated actin monomers at pH 8.0 is thermodynamically less stable than the ones obtained from ATP-actin monomers.

Original languageEnglish
Pages (from-to)619-623
Number of pages5
JournalJournal of Thermal Analysis and Calorimetry
Volume84
Issue number3
DOIs
Publication statusPublished - May 2006

Fingerprint

adenosine triphosphate
Adenosinetriphosphate
Administrative data processing
adenosine diphosphate
Actins
filaments
monomers
Monomers
Adenosine Diphosphate
Enthalpy
Adenosine Triphosphate
enthalpy
heat
Denaturation
biopolymer denaturation
Magnesium
melting points
Melting point
Hot Temperature
magnesium

Keywords

  • Actin
  • Denaturation
  • DSC
  • Nucleotides
  • Thermodynamics

ASJC Scopus subject areas

  • Chemical Engineering(all)

Cite this

Thermal characterisation of actin filaments prepared from adp-actin monomers. / Orbán, J.; Pozsonyi, Kinga; Szarka, Krisztina; Barkó, Szilvia; Bódis, Emoke; Lőrinczy, D.

In: Journal of Thermal Analysis and Calorimetry, Vol. 84, No. 3, 05.2006, p. 619-623.

Research output: Contribution to journalArticle

Orbán, J. ; Pozsonyi, Kinga ; Szarka, Krisztina ; Barkó, Szilvia ; Bódis, Emoke ; Lőrinczy, D. / Thermal characterisation of actin filaments prepared from adp-actin monomers. In: Journal of Thermal Analysis and Calorimetry. 2006 ; Vol. 84, No. 3. pp. 619-623.
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AU - Lőrinczy, D.

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