Thermal analysis in biological and medical applications

Research output: Contribution to journalReview article

5 Citations (Scopus)

Abstract

In this paper, I will sum up our research activity from this field performed in the last about 25 years. I will focus on three main points: basic muscle research in the different intermediate states of ATP hydrolysis cycle during muscle contraction, R&D activities to develop and test different dairy products and TA application in some surgical and diagnostic problem. Our initial research concerned the investigation of thermal stability of main muscle proteins clarifies their basic unfolding characteristics. Later we extended the thermal stability investigation from protein solution to the myosin myofibrils and to the higher organization of muscle proteins, the muscle fibres, checking the effect of nucleotides. At that time, it became possible to stabilize the different intermediate states of ATP hydrolysis up to the time of DSC measurement, using different Pi analogues (Vi, AlFx and BeFx) and non-hydrolysable ATP analogue (AMP.PNP). This way the targets were AM.ADP.Vi (and with AlFx or BeFx) so-called weak binding state, AM.ADP the “strong” binding state as well as the “rigor” AM complex state. AM.AMP.PNP state was used to mimic the AM.ATP state. With our R&D cooperation, a cold spreadable butter was successfully developed. We were a partner in the development of Ca-enriched cheese, in its spreadable form too as well as in the development and testing of different dairy products using probiotic cultures. Our TA activity covers a wide range of medical applications, e.g. investigation of the different abnormalities of human leg skeletal muscle, different stages of degeneration of human vertebral disc, to judge the goodness/applicability of different suture technique on tracheal cartilage in primary airway reconstruction or the characterization of different self-expandable stents implantation in the oesophagus treatment. We have joined those groups who try to use DSC in the diagnosis of different diseases from blood plasma, e.g. in the case of breast cancer, melanoma, in psoriasis.

Original languageEnglish
Pages (from-to)1263-1280
Number of pages18
JournalJournal of Thermal Analysis and Calorimetry
Volume130
Issue number3
DOIs
Publication statusPublished - Dec 1 2017

Fingerprint

Medical applications
Thermoanalysis
adenosine triphosphate
Muscle
thermal analysis
Adenosine Triphosphate
Dairy products
muscles
Muscle Proteins
adenosine monophosphate
adenosine diphosphate
Adenosine Diphosphate
Hydrolysis
proteins
Thermodynamic stability
Adenylyl Imidodiphosphate
hydrolysis
thermal stability
Cheeses
Stents

Keywords

  • Actin/myosin
  • ATP hydrolysis cycle
  • Blood plasma
  • Butter
  • Cancer
  • Cheese
  • DSC
  • Probiotic dairy products
  • Tendons/cartilages

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Physical and Theoretical Chemistry

Cite this

Thermal analysis in biological and medical applications. / Lőrinczy, D.

In: Journal of Thermal Analysis and Calorimetry, Vol. 130, No. 3, 01.12.2017, p. 1263-1280.

Research output: Contribution to journalReview article

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