Theory of electric signals of membrane proteins in three dimensions

László Oroszi, A. Dér, P. Ormos

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Transmembrane ion pumps are often investigated experimentally by photoelectric measurements in model systems. In addition to the most widely used systems based on model membranes, a fundamentally different class is represented by the so-called suspension methods. In this technique the electric signal is measured on a bulk suspension of oriented ion pumps in the form of a displacement current. On this system, electric and spectroscopic experiments can be performed simultaneously. Using the information from both types of measurements, and utilizing the three-dimensional nature of the system, it is possible to follow the intramolecular charge motions in all three spatial directions. The derivable dipole moment changes associated with conformational transitions allow the verification of molecular dynamic models. In this work a theory is presented to describe the suspension method; samples with different symmetry properties and the possibilities of photoselection to obtain the desired three-dimensional information are analyzed.

Original languageEnglish
Pages (from-to)136-144
Number of pages9
JournalEuropean Biophysics Journal
Volume31
Issue number2
DOIs
Publication statusPublished - May 2002

Fingerprint

Ion Pumps
Suspensions
Membrane Proteins
Molecular Models
Molecular Dynamics Simulation
Membranes

Keywords

  • Conformational changes
  • Electric signals
  • Membrane proteins
  • Photoselection
  • Three-dimensional systems

ASJC Scopus subject areas

  • Biophysics

Cite this

Theory of electric signals of membrane proteins in three dimensions. / Oroszi, László; Dér, A.; Ormos, P.

In: European Biophysics Journal, Vol. 31, No. 2, 05.2002, p. 136-144.

Research output: Contribution to journalArticle

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