Fehérjechipek elméleti alapjai és alkalmazási lehetoségük az orvosi diagnosztikában és kutatásban

Translated title of the contribution: Theoretical foundations of protein chips and their possible use in medical research and diagnostics

Research output: Contribution to journalReview article

2 Citations (Scopus)

Abstract

The confirmation of mRNA expression studies by protein chips is of high recent interest due to the widespread application of expression arrays. In this review the advantages, technical limitations, application fields and the first results of the protein arrays is described. The bottlenecks of the increasing protein array applications are the fast decomposition of proteins, the problem with aspecific binding and the lack of amplification techniques. Today glass slide based printed, SELDI (MS) based, elektrophoresis based and tissue microarray based technologies are available. The advantage of the glass slide based chips are the simplicity of their application, and relatively low cost. The SELDI based protein chip technique is applicable to minute amounts of starting material (<1 μg) but it is the most expensive one. The electrophoresis based techniques are still under intensive development. The tissue microarrays can be used for the parallel testing of the sensitivity and specificity of single antibodies on a broad range of histological specimens on a single slide. Protein chips were successfully used for serum tumor marker detection, cancer research, cell physiology studies and for the verification of mRNA expression studies. Protein chips are envisioned to be available for routine diagnostic applications if the ongoing technology development will be successful in increase in sensitivity, specificity, costs reduction and for the reduction of the necessery sample volume.

Translated title of the contributionTheoretical foundations of protein chips and their possible use in medical research and diagnostics
Original languageHungarian
Pages (from-to)1511-1520
Number of pages10
JournalOrvosi hetilap
Volume148
Issue number32
DOIs
Publication statusPublished - Aug 12 2007

ASJC Scopus subject areas

  • Medicine(all)

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