Fehérjechipek elméleti alapjai és alkalmazási lehetoségük az orvosi diagnosztikában és kutatásban

Translated title of the contribution: Theoretical foundations of protein chips and their possible use in medical research and diagnostics

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The confirmation of mRNA expression studies by protein chips is of high recent interest due to the widespread application of expression arrays. In this review the advantages, technical limitations, application fields and the first results of the protein arrays is described. The bottlenecks of the increasing protein array applications are the fast decomposition of proteins, the problem with aspecific binding and the lack of amplification techniques. Today glass slide based printed, SELDI (MS) based, elektrophoresis based and tissue microarray based technologies are available. The advantage of the glass slide based chips are the simplicity of their application, and relatively low cost. The SELDI based protein chip technique is applicable to minute amounts of starting material (

Original languageHungarian
Pages (from-to)1511-1520
Number of pages10
JournalOrvosi Hetilap
Volume148
Issue number32
DOIs
Publication statusPublished - Aug 12 2007

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Protein Array Analysis
Biomedical Research
Glass
Technology
Costs and Cost Analysis
Messenger RNA
Proteins

ASJC Scopus subject areas

  • Medicine(all)

Cite this

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title = "Feh{\'e}rjechipek elm{\'e}leti alapjai {\'e}s alkalmaz{\'a}si lehetos{\'e}g{\"u}k az orvosi diagnosztik{\'a}ban {\'e}s kutat{\'a}sban",
abstract = "The confirmation of mRNA expression studies by protein chips is of high recent interest due to the widespread application of expression arrays. In this review the advantages, technical limitations, application fields and the first results of the protein arrays is described. The bottlenecks of the increasing protein array applications are the fast decomposition of proteins, the problem with aspecific binding and the lack of amplification techniques. Today glass slide based printed, SELDI (MS) based, elektrophoresis based and tissue microarray based technologies are available. The advantage of the glass slide based chips are the simplicity of their application, and relatively low cost. The SELDI based protein chip technique is applicable to minute amounts of starting material (",
keywords = "Chip technol{\'o}gy, Proteins, Proteomics, Serum disease profile, Signal transduction pathway analysis",
author = "S. Spis{\'a}k and B. Moln{\'a}r and O. Galamb and F. S{\'i}pos and Z. Tulassay",
year = "2007",
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language = "Hungarian",
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publisher = "Akademiai Kiado",
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T1 - Fehérjechipek elméleti alapjai és alkalmazási lehetoségük az orvosi diagnosztikában és kutatásban

AU - Spisák, S.

AU - Molnár, B.

AU - Galamb, O.

AU - Sípos, F.

AU - Tulassay, Z.

PY - 2007/8/12

Y1 - 2007/8/12

N2 - The confirmation of mRNA expression studies by protein chips is of high recent interest due to the widespread application of expression arrays. In this review the advantages, technical limitations, application fields and the first results of the protein arrays is described. The bottlenecks of the increasing protein array applications are the fast decomposition of proteins, the problem with aspecific binding and the lack of amplification techniques. Today glass slide based printed, SELDI (MS) based, elektrophoresis based and tissue microarray based technologies are available. The advantage of the glass slide based chips are the simplicity of their application, and relatively low cost. The SELDI based protein chip technique is applicable to minute amounts of starting material (

AB - The confirmation of mRNA expression studies by protein chips is of high recent interest due to the widespread application of expression arrays. In this review the advantages, technical limitations, application fields and the first results of the protein arrays is described. The bottlenecks of the increasing protein array applications are the fast decomposition of proteins, the problem with aspecific binding and the lack of amplification techniques. Today glass slide based printed, SELDI (MS) based, elektrophoresis based and tissue microarray based technologies are available. The advantage of the glass slide based chips are the simplicity of their application, and relatively low cost. The SELDI based protein chip technique is applicable to minute amounts of starting material (

KW - Chip technológy

KW - Proteins

KW - Proteomics

KW - Serum disease profile

KW - Signal transduction pathway analysis

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