THEORETICAL AND PRACTICAL ASPECTS OF IMMOBILIZED MULTI-STEP ENZYME SYSTEMS.

K. Mosbach, B. Mattiasson, S. Gestrelius, P. Srere

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

This paper describes a new approach to the utilization of enzyme system in which a sequence of enzymes is immobilized. The two enzymes (hexokinase and glucose-6-phosphate dehydrogenase) were immobilized together onto Sepharose beads using the cyanogen bromide method. The efficiency (product/time) of such an enzyme system was compared to either the corresponding soluble enzyme system or to preparations where the two enzymes had been bound to separate beads. It was found that the first mentioned matrix-bound system reached steady-state conditions much faster than the two other systems, both of which showed a very pronounced lag phase. Theoretical considerations are followed by a description of equipment used in the technical processes involving multi-step enzyme catalysis.

Original languageEnglish
Title of host publicationUnknown Host Publication Title
PublisherPlenum Press (v 2)
Pages143-152
Number of pages10
Publication statusPublished - 1974
EventEng Found Conf on Enzyme Eng, 2nd, Proc - Henniker, NH, USA
Duration: Aug 5 1973Aug 10 1973

Other

OtherEng Found Conf on Enzyme Eng, 2nd, Proc
CityHenniker, NH, USA
Period8/5/738/10/73

Fingerprint

Enzymes
Catalysis
Glucose
Phosphates

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Mosbach, K., Mattiasson, B., Gestrelius, S., & Srere, P. (1974). THEORETICAL AND PRACTICAL ASPECTS OF IMMOBILIZED MULTI-STEP ENZYME SYSTEMS. In Unknown Host Publication Title (pp. 143-152). Plenum Press (v 2).

THEORETICAL AND PRACTICAL ASPECTS OF IMMOBILIZED MULTI-STEP ENZYME SYSTEMS. / Mosbach, K.; Mattiasson, B.; Gestrelius, S.; Srere, P.

Unknown Host Publication Title. Plenum Press (v 2), 1974. p. 143-152.

Research output: Chapter in Book/Report/Conference proceedingChapter

Mosbach, K, Mattiasson, B, Gestrelius, S & Srere, P 1974, THEORETICAL AND PRACTICAL ASPECTS OF IMMOBILIZED MULTI-STEP ENZYME SYSTEMS. in Unknown Host Publication Title. Plenum Press (v 2), pp. 143-152, Eng Found Conf on Enzyme Eng, 2nd, Proc, Henniker, NH, USA, 8/5/73.
Mosbach K, Mattiasson B, Gestrelius S, Srere P. THEORETICAL AND PRACTICAL ASPECTS OF IMMOBILIZED MULTI-STEP ENZYME SYSTEMS. In Unknown Host Publication Title. Plenum Press (v 2). 1974. p. 143-152
Mosbach, K. ; Mattiasson, B. ; Gestrelius, S. ; Srere, P. / THEORETICAL AND PRACTICAL ASPECTS OF IMMOBILIZED MULTI-STEP ENZYME SYSTEMS. Unknown Host Publication Title. Plenum Press (v 2), 1974. pp. 143-152
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