Theoretical advantages and drawbacks of on-line, multidimensional liquid chromatography using multiple columns operated in parallel

Jacob N. Fairchild, Krisztián Horváth, Georges Guiochon

Research output: Contribution to journalArticle

28 Citations (Scopus)


The theoretical advantages and drawbacks of using a multiple-, parallel column approach in on-line multidimensional liquid chromatography systems were investigated. Much time or peak capacity can be gained with the use of multiple parallel columns at the second-dimension while the aggregate time of separation increases only by the increment of the gradient time of the second-dimension. Multidimensional chromatographic systems are now used to perform many tasks ranging from routine, fast analyses to specialized, arduous separations. In this work, we focus on the advantages of a multiple, parallel columns approach to on-line multidimensional liquid chromatography systems. Calculations of the achievable peak capacities were made as functions of the number of columns operated in parallel. Increasing the number of second-dimension columns from one to two or three causes the largest increase in peak capacity with only a slight increase of aggregate time. We also present some practical aspects to consider when attempting multidimensional separations with multiple columns operated in parallel.

Original languageEnglish
Pages (from-to)6210-6217
Number of pages8
JournalJournal of Chromatography A
Issue number34
Publication statusPublished - Aug 21 2009



  • Multidimensional chromatography
  • Parallel columns
  • Two-dimensional liquid chromatography

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Cite this