Theoretical advantages and drawbacks of on-line, multidimensional liquid chromatography using multiple columns operated in parallel

Jacob N. Fairchild, K. Horváth, Georges Guiochon

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The theoretical advantages and drawbacks of using a multiple-, parallel column approach in on-line multidimensional liquid chromatography systems were investigated. Much time or peak capacity can be gained with the use of multiple parallel columns at the second-dimension while the aggregate time of separation increases only by the increment of the gradient time of the second-dimension. Multidimensional chromatographic systems are now used to perform many tasks ranging from routine, fast analyses to specialized, arduous separations. In this work, we focus on the advantages of a multiple, parallel columns approach to on-line multidimensional liquid chromatography systems. Calculations of the achievable peak capacities were made as functions of the number of columns operated in parallel. Increasing the number of second-dimension columns from one to two or three causes the largest increase in peak capacity with only a slight increase of aggregate time. We also present some practical aspects to consider when attempting multidimensional separations with multiple columns operated in parallel.

Original languageEnglish
Pages (from-to)6210-6217
Number of pages8
JournalJournal of Chromatography A
Volume1216
Issue number34
DOIs
Publication statusPublished - Aug 21 2009

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Liquid chromatography
Liquid Chromatography

Keywords

  • Multidimensional chromatography
  • Parallel columns
  • Two-dimensional liquid chromatography

ASJC Scopus subject areas

  • Analytical Chemistry
  • Organic Chemistry
  • Biochemistry

Cite this

Theoretical advantages and drawbacks of on-line, multidimensional liquid chromatography using multiple columns operated in parallel. / Fairchild, Jacob N.; Horváth, K.; Guiochon, Georges.

In: Journal of Chromatography A, Vol. 1216, No. 34, 21.08.2009, p. 6210-6217.

Research output: Contribution to journalArticle

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