Theophylline infusion modulates prostaglandin and leukotriene production in man

T. Kerttula, S. Kaukinen, E. Seppälä, A. Riutta, I. Muchá, P. Ylitalo, J. Alanko

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Although theophylline has been used in the treatment of asthma for decades, it is not a first line choice any more, It is a well-known bronchodilator, but was recently discovered also to be an anti-inflammatory, immunomodulatory and bronchoprotective agent. Therefore we wanted to establish the role of theophylline on prostaglandin and leukotriene production, which plays a part in the pathogenesis of asthma. Theophylline was infused (bolus 5 mg/kg in 15 min and infusion 0.4 mg/kg/h for 1 h 45 min) into healthy volunteers. Thromboxane B2, prostaglandin E2 and leukotriene E4 were measured from the A23187-stimulated whole blood samples and stable metabolites of thromboxane A2; prostacyclin and leukotriene E4 were measured from urine. Theophylline increased prostaglandin E2 production and decreased leukotriene E4 production ex vivo in whole blood, thus increasing the prostanoid/leukotriene ratio. It did not change thromboxane B2 production stimulated by either spontaneous clotting or A23187 in the whole blood. Theophylline had hardly any effect on in vivo thromboxane, prostacyclin and leukotriene E4 production measured as urinary metabolites, 11-dehydro-thromboxane B2, 2,3-dinor-6-keto-prostaglandin F(1α) and leukotriene E4, respectively. Serum theophylline concentrations were at the lower level of normal therapeutic range during the infusion. The increase in PGE2 and the decrease in LTE4 synthesis ex vivo may offer a new explanation for the mode of antiasthmatic action of theophylline. It is notable that this phenomenon occurs at low serum theophylline concentrations. These results confirm the idea that theophylline has an anti-inflammatory and bronchoprotective action and support the use of theophylline as a therapeutic agent in asthmatic patients.

Original languageEnglish
Pages (from-to)555-560
Number of pages6
JournalProstaglandins Leukotrienes and Essential Fatty Acids
Volume57
Issue number6
Publication statusPublished - Dec 1997

Fingerprint

Leukotrienes
Theophylline
Prostaglandins
Leukotriene E4
Dinoprostone
Thromboxane B2
Blood
Calcimycin
Epoprostenol
Metabolites
Anti-Inflammatory Agents
Asthma
Anti-Asthmatic Agents
Thromboxane A2
Thromboxanes
Bronchodilator Agents
Prostaglandins F
Serum
Healthy Volunteers
Reference Values

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Kerttula, T., Kaukinen, S., Seppälä, E., Riutta, A., Muchá, I., Ylitalo, P., & Alanko, J. (1997). Theophylline infusion modulates prostaglandin and leukotriene production in man. Prostaglandins Leukotrienes and Essential Fatty Acids, 57(6), 555-560.

Theophylline infusion modulates prostaglandin and leukotriene production in man. / Kerttula, T.; Kaukinen, S.; Seppälä, E.; Riutta, A.; Muchá, I.; Ylitalo, P.; Alanko, J.

In: Prostaglandins Leukotrienes and Essential Fatty Acids, Vol. 57, No. 6, 12.1997, p. 555-560.

Research output: Contribution to journalArticle

Kerttula, T, Kaukinen, S, Seppälä, E, Riutta, A, Muchá, I, Ylitalo, P & Alanko, J 1997, 'Theophylline infusion modulates prostaglandin and leukotriene production in man', Prostaglandins Leukotrienes and Essential Fatty Acids, vol. 57, no. 6, pp. 555-560.
Kerttula, T. ; Kaukinen, S. ; Seppälä, E. ; Riutta, A. ; Muchá, I. ; Ylitalo, P. ; Alanko, J. / Theophylline infusion modulates prostaglandin and leukotriene production in man. In: Prostaglandins Leukotrienes and Essential Fatty Acids. 1997 ; Vol. 57, No. 6. pp. 555-560.
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