Theileria highjacks JNK2 into a complex with the macroschizont GPI (GlycosylPhosphatidylInositol)-anchored surface protein p104

Perle Latré De Laté, Malak Haidar, Hifzur Ansari, Shahin Tajeri, Eszter Szarka, Anita Alexa, Kerry Woods, A. Reményi, Arnab Pain, Gordon Langsley

Research output: Contribution to journalArticle

Abstract

Constitutive c-Jun N-terminal kinase (JNK) activity characterizes bovine T and B cells infected with Theileria parva, and B cells and macrophages infected with Theileria annulata. Here, we show that T. annulata infection of macrophages manipulates JNK activation by recruiting JNK2 and not JNK1 to the parasite surface, whereas JNK1 is found predominantly in the host cell nucleus. At the parasite's surface, JNK2 forms a complex with p104, a GPI-(GlycosylPhosphatidylInositol)-anchor T. annulata plasma membrane protein. Sequestration of JNK2 depended on Protein Kinase-A (PKA)-mediated phosphorylation of a JNK-binding motif common to T. parva and a cell penetrating peptide harbouring the conserved p104 JNK-binding motif competitively ablated binding, whereupon liberated JNK2 became ubiquitinated and degraded. Cytosolic sequestration of JNK2 suppressed small mitochondrial ARF-mediated autophagy, whereas it sustained nuclear JNK1 levels, c-Jun phosphorylation, and matrigel traversal. Therefore, T. annulata sequestration of JNK2 contributes to both survival and dissemination of Theileria-transformed macrophages.

Original languageEnglish
Article numbere12973
JournalCellular Microbiology
DOIs
Publication statusAccepted/In press - Jan 1 2018

Fingerprint

Theileria
Glycosylphosphatidylinositols
JNK Mitogen-Activated Protein Kinases
Membrane Proteins
Macrophages
Parasites
B-Lymphocytes
Theileria annulata
Theileria parva
Phosphorylation
Cell-Penetrating Peptides
Autophagy
Cyclic AMP-Dependent Protein Kinases
Cell Nucleus
Blood Proteins
Cell Membrane
T-Lymphocytes
Infection

Keywords

  • Dissemination
  • JNK2
  • penetrating peptide
  • PKA
  • Theileria

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

Cite this

Latré De Laté, P., Haidar, M., Ansari, H., Tajeri, S., Szarka, E., Alexa, A., ... Langsley, G. (Accepted/In press). Theileria highjacks JNK2 into a complex with the macroschizont GPI (GlycosylPhosphatidylInositol)-anchored surface protein p104. Cellular Microbiology, [e12973]. https://doi.org/10.1111/cmi.12973

Theileria highjacks JNK2 into a complex with the macroschizont GPI (GlycosylPhosphatidylInositol)-anchored surface protein p104. / Latré De Laté, Perle; Haidar, Malak; Ansari, Hifzur; Tajeri, Shahin; Szarka, Eszter; Alexa, Anita; Woods, Kerry; Reményi, A.; Pain, Arnab; Langsley, Gordon.

In: Cellular Microbiology, 01.01.2018.

Research output: Contribution to journalArticle

Latré De Laté, Perle ; Haidar, Malak ; Ansari, Hifzur ; Tajeri, Shahin ; Szarka, Eszter ; Alexa, Anita ; Woods, Kerry ; Reményi, A. ; Pain, Arnab ; Langsley, Gordon. / Theileria highjacks JNK2 into a complex with the macroschizont GPI (GlycosylPhosphatidylInositol)-anchored surface protein p104. In: Cellular Microbiology. 2018.
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