The VELVET a orthologue VEL1 of trichoderma reesei regulates fungal development and is essential for cellulase gene expression

Razieh Karimi Aghcheh, Zoltán Németh, Lea Atanasova, E. Fekete, Melinda Paholcsek, E. Sándor, Benigno Aquino, Irina S. Druzhinina, L. Karaffa, Christian P. Kubicek

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Trichoderma reesei is the industrial producer of cellulases and hemicellulases for biorefinery processes. Their expression is obligatorily dependent on the function of the protein methyltransferase LAE1. The Aspergillus nidulans orthologue of LAE1-LaeA-is part of the VELVET protein complex consisting of LaeA, VeA and VelB that regulates secondary metabolism and sexual as well as asexual reproduction. Here we have therefore investigated the function of VEL1, the T. reesei orthologue of A. nidulans VeA. Deletion of the T. reesei vel1 locus causes a complete and light-independent loss of conidiation, and impairs formation of perithecia. Deletion of vel1 also alters hyphal morphology towards hyperbranching and formation of thicker filaments, and with consequently reduced growth rates. Growth on lactose as a sole carbon source, however, is even more strongly reduced and growth on cellulose as a sole carbon source eliminated. Consistent with these findings, deletion of vel1 completely impaired the expression of cellulases, xylanases and the cellulase regulator XYR1 on lactose as a cellulase inducing carbon source, but also in resting mycelia with sophorose as inducer. Our data show that in T. reesei VEL1 controls sexual and asexual development, and this effect is independent of light. VEL1 is also essential for cellulase gene expression, which is consistent with the assumption that their regulation by LAE1 occurs by the VELVET complex.

Original languageEnglish
Article numbere112799
JournalPLoS One
Volume9
Issue number11
DOIs
Publication statusPublished - Nov 11 2014

Fingerprint

Trichoderma reesei
Trichoderma
Cellulase
Essential Genes
endo-1,4-beta-glucanase
Gene expression
Cellulases
Aspergillus nidulans
Carbon
Lactose
Gene Expression
gene expression
cellulases
Growth
Protein Methyltransferases
Asexual Reproduction
Secondary Metabolism
lactose
Light
carbon

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

The VELVET a orthologue VEL1 of trichoderma reesei regulates fungal development and is essential for cellulase gene expression. / Aghcheh, Razieh Karimi; Németh, Zoltán; Atanasova, Lea; Fekete, E.; Paholcsek, Melinda; Sándor, E.; Aquino, Benigno; Druzhinina, Irina S.; Karaffa, L.; Kubicek, Christian P.

In: PLoS One, Vol. 9, No. 11, e112799, 11.11.2014.

Research output: Contribution to journalArticle

Aghcheh, Razieh Karimi ; Németh, Zoltán ; Atanasova, Lea ; Fekete, E. ; Paholcsek, Melinda ; Sándor, E. ; Aquino, Benigno ; Druzhinina, Irina S. ; Karaffa, L. ; Kubicek, Christian P. / The VELVET a orthologue VEL1 of trichoderma reesei regulates fungal development and is essential for cellulase gene expression. In: PLoS One. 2014 ; Vol. 9, No. 11.
@article{aab868d27aba42e89706109ed1d84a54,
title = "The VELVET a orthologue VEL1 of trichoderma reesei regulates fungal development and is essential for cellulase gene expression",
abstract = "Trichoderma reesei is the industrial producer of cellulases and hemicellulases for biorefinery processes. Their expression is obligatorily dependent on the function of the protein methyltransferase LAE1. The Aspergillus nidulans orthologue of LAE1-LaeA-is part of the VELVET protein complex consisting of LaeA, VeA and VelB that regulates secondary metabolism and sexual as well as asexual reproduction. Here we have therefore investigated the function of VEL1, the T. reesei orthologue of A. nidulans VeA. Deletion of the T. reesei vel1 locus causes a complete and light-independent loss of conidiation, and impairs formation of perithecia. Deletion of vel1 also alters hyphal morphology towards hyperbranching and formation of thicker filaments, and with consequently reduced growth rates. Growth on lactose as a sole carbon source, however, is even more strongly reduced and growth on cellulose as a sole carbon source eliminated. Consistent with these findings, deletion of vel1 completely impaired the expression of cellulases, xylanases and the cellulase regulator XYR1 on lactose as a cellulase inducing carbon source, but also in resting mycelia with sophorose as inducer. Our data show that in T. reesei VEL1 controls sexual and asexual development, and this effect is independent of light. VEL1 is also essential for cellulase gene expression, which is consistent with the assumption that their regulation by LAE1 occurs by the VELVET complex.",
author = "Aghcheh, {Razieh Karimi} and Zolt{\'a}n N{\'e}meth and Lea Atanasova and E. Fekete and Melinda Paholcsek and E. S{\'a}ndor and Benigno Aquino and Druzhinina, {Irina S.} and L. Karaffa and Kubicek, {Christian P.}",
year = "2014",
month = "11",
day = "11",
doi = "10.1371/journal.pone.0112799",
language = "English",
volume = "9",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "11",

}

TY - JOUR

T1 - The VELVET a orthologue VEL1 of trichoderma reesei regulates fungal development and is essential for cellulase gene expression

AU - Aghcheh, Razieh Karimi

AU - Németh, Zoltán

AU - Atanasova, Lea

AU - Fekete, E.

AU - Paholcsek, Melinda

AU - Sándor, E.

AU - Aquino, Benigno

AU - Druzhinina, Irina S.

AU - Karaffa, L.

AU - Kubicek, Christian P.

PY - 2014/11/11

Y1 - 2014/11/11

N2 - Trichoderma reesei is the industrial producer of cellulases and hemicellulases for biorefinery processes. Their expression is obligatorily dependent on the function of the protein methyltransferase LAE1. The Aspergillus nidulans orthologue of LAE1-LaeA-is part of the VELVET protein complex consisting of LaeA, VeA and VelB that regulates secondary metabolism and sexual as well as asexual reproduction. Here we have therefore investigated the function of VEL1, the T. reesei orthologue of A. nidulans VeA. Deletion of the T. reesei vel1 locus causes a complete and light-independent loss of conidiation, and impairs formation of perithecia. Deletion of vel1 also alters hyphal morphology towards hyperbranching and formation of thicker filaments, and with consequently reduced growth rates. Growth on lactose as a sole carbon source, however, is even more strongly reduced and growth on cellulose as a sole carbon source eliminated. Consistent with these findings, deletion of vel1 completely impaired the expression of cellulases, xylanases and the cellulase regulator XYR1 on lactose as a cellulase inducing carbon source, but also in resting mycelia with sophorose as inducer. Our data show that in T. reesei VEL1 controls sexual and asexual development, and this effect is independent of light. VEL1 is also essential for cellulase gene expression, which is consistent with the assumption that their regulation by LAE1 occurs by the VELVET complex.

AB - Trichoderma reesei is the industrial producer of cellulases and hemicellulases for biorefinery processes. Their expression is obligatorily dependent on the function of the protein methyltransferase LAE1. The Aspergillus nidulans orthologue of LAE1-LaeA-is part of the VELVET protein complex consisting of LaeA, VeA and VelB that regulates secondary metabolism and sexual as well as asexual reproduction. Here we have therefore investigated the function of VEL1, the T. reesei orthologue of A. nidulans VeA. Deletion of the T. reesei vel1 locus causes a complete and light-independent loss of conidiation, and impairs formation of perithecia. Deletion of vel1 also alters hyphal morphology towards hyperbranching and formation of thicker filaments, and with consequently reduced growth rates. Growth on lactose as a sole carbon source, however, is even more strongly reduced and growth on cellulose as a sole carbon source eliminated. Consistent with these findings, deletion of vel1 completely impaired the expression of cellulases, xylanases and the cellulase regulator XYR1 on lactose as a cellulase inducing carbon source, but also in resting mycelia with sophorose as inducer. Our data show that in T. reesei VEL1 controls sexual and asexual development, and this effect is independent of light. VEL1 is also essential for cellulase gene expression, which is consistent with the assumption that their regulation by LAE1 occurs by the VELVET complex.

UR - http://www.scopus.com/inward/record.url?scp=84914695508&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84914695508&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0112799

DO - 10.1371/journal.pone.0112799

M3 - Article

C2 - 25386652

AN - SCOPUS:84914695508

VL - 9

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 11

M1 - e112799

ER -