The value of MALDI-TOF MS for the identification of clinically relevant anaerobic bacteria in routine laboratories

E. Nagy, Simone Becker, Markus Kostrzewa, Noémi Barta, E. Urbán

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Abstract

Between 2010 and 2011, 283 clinically relevant non-duplicate anaerobic isolates were analysed by MALDI-TOF MS and the results were compared with conventional identification. Immediately after isolation, an ethanol precipitation was carried out on isolated colonies and the stabilized samples were anonymized and sent to the laboratory of Bruker Daltonik, Bremen, Germany, where the identification was done using the standard protocol for micro-organism identification on a Microflex LT mass spectrometer equipped with the MALDI Biotyper 3.0 software. Of 283 isolates, 218 (77 %) were identified at species level [log(score) ≥2.0], 31 isolates (10.95 %) were identified at genus level [log(score) 1.7-2.0] and 34 (12 %) gave non-reliable identification [log(score)

Original languageEnglish
Pages (from-to)1393-1400
Number of pages8
JournalJournal of Medical Microbiology
Volume61
Issue numberPART 10
DOIs
Publication statusPublished - Oct 2012

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Anaerobic Bacteria
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Germany
Ethanol
Software

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

The value of MALDI-TOF MS for the identification of clinically relevant anaerobic bacteria in routine laboratories. / Nagy, E.; Becker, Simone; Kostrzewa, Markus; Barta, Noémi; Urbán, E.

In: Journal of Medical Microbiology, Vol. 61, No. PART 10, 10.2012, p. 1393-1400.

Research output: Contribution to journalArticle

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