The vacuolar Ca2+/H+ exchanger Vcx1p/Hum1p tightly controls cytosolic Ca2+ levels in S. cerevisiae

Attila Miseta, Richard Kellermayer, David P. Aiello, Lianwu Fu, David M. Bedwell

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106 Citations (Scopus)


It is well established that the vacuole plays an important role in the cellular adaptation to growth in the presence of elevated extracellular Ca2+ concentrations in Saccharomyces cerevisiae. The Ca2+ ATPase Pmc1p and the Ca2+/H+ exchanger Vcx1p/Hum1p have been shown to facilitate Ca2+ sequestration into the vacuole. However, the distinct physiological roles of these two vacuolar Ca2+ transporters remain uncertain. Here we show that Vcx1p can rapidly sequester a sudden pulse of cytosolic Ca2+ into the vacuole, while Pmc1p carries out this function much less efficiently. This finding is consistent with the postulated role of Vcx1p as a high capacity, low affinity Ca2+ transporter and suggests that Vcx1p may act to attenuate the propagation of Ca2+ signals in this organism. Copyright (C) 1999 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)132-136
Number of pages5
JournalFEBS letters
Issue number2
Publication statusPublished - May 21 1999



  • Aequorin
  • Ca ATPase
  • Ca/H exchanger
  • Vacuole
  • Yeast

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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