The vacuolar Ca2+/H+ exchanger Vcx1p/Hum1p tightly controls cytosolic Ca2+ levels in S. cerevisiae

Attila Miseta, Richard Kellermayer, David P. Aiello, Lianwu Fu, David M. Bedwell

Research output: Contribution to journalArticle

106 Citations (Scopus)

Abstract

It is well established that the vacuole plays an important role in the cellular adaptation to growth in the presence of elevated extracellular Ca2+ concentrations in Saccharomyces cerevisiae. The Ca2+ ATPase Pmc1p and the Ca2+/H+ exchanger Vcx1p/Hum1p have been shown to facilitate Ca2+ sequestration into the vacuole. However, the distinct physiological roles of these two vacuolar Ca2+ transporters remain uncertain. Here we show that Vcx1p can rapidly sequester a sudden pulse of cytosolic Ca2+ into the vacuole, while Pmc1p carries out this function much less efficiently. This finding is consistent with the postulated role of Vcx1p as a high capacity, low affinity Ca2+ transporter and suggests that Vcx1p may act to attenuate the propagation of Ca2+ signals in this organism. Copyright (C) 1999 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)132-136
Number of pages5
JournalFEBS letters
Volume451
Issue number2
DOIs
Publication statusPublished - May 21 1999

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Keywords

  • Aequorin
  • Ca ATPase
  • Ca/H exchanger
  • Vacuole
  • Yeast

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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