The usefulness of in vivo gene expression investigations from peripheral white blood cells: A preliminary study

I. Ember, I. Kiss, T. Raposa

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Alterations of onco/tumour suppressor genes are involved in the formation of human hematological malignancies. Previously, in animal models, we demonstrated the applicability of in vivo gene expression investigations to monitor the effects of certain carcinogenic chemicals. In our present study we determined the expression of onco/suppressor genes from isolated peripheral white blood cells of patients with chronic myeloid leukaemia (CLL) and non-Hodgkin lymphoma (NHL). Gene expressions were determined by isolation of total RNA and slot blot hybridization with chemiluminescently labeled gene probes (Ha-ras, c-myc and p53). Expression levels were compared before and after treatment with a combined cytostatic protocol, containing cyclophosphamide, doxorubicin, vincristine and prednisolone (CHOP). Both the CLL and NHL group of patients exhibited significantly higher expression of the investigated genes than healthy controls. One month after the cytostatic treatment, we found considerably fewer individuals with overexpressed oncogenes than before the treatment. Our study proved that onco/tumour suppressor gene expressions could be used as biomarkers of certain hematological malignancies, and to monitor the therapeutical effect of cytostatic drugs.

Original languageEnglish
Pages (from-to)331-334
Number of pages4
JournalEuropean Journal of Cancer Prevention
Volume8
Issue number4
Publication statusPublished - 1999

Fingerprint

Tumor Suppressor Genes
Leukocytes
Gene Expression
Cytostatic Agents
Hematologic Neoplasms
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Non-Hodgkin's Lymphoma
ras Genes
Vincristine
Prednisolone
Oncogenes
Doxorubicin
Cyclophosphamide
Therapeutics
Animal Models
Biomarkers
RNA

Keywords

  • Cytostatic treatment
  • Gene expression
  • Leukaemia
  • Lymphoma
  • Oncogene
  • Tumour suppressor gene

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

The usefulness of in vivo gene expression investigations from peripheral white blood cells : A preliminary study. / Ember, I.; Kiss, I.; Raposa, T.

In: European Journal of Cancer Prevention, Vol. 8, No. 4, 1999, p. 331-334.

Research output: Contribution to journalArticle

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