The use of planar electrophoresis for detection of single-nucleotide polymorphism

Krisztina Boór, Z. Rónai, M. Sasvári, Péter Gaszner, H. Kalász

Research output: Contribution to journalArticle

Abstract

The polymorphic human DNA sequence in the promoter region of the dopamine D4 receptor gene has been investigated by means of non-invasive DNA sampling, amplification by means of the polymerase chain reaction, and subsequent separation of the reaction products by horizontal slab-gel electrophoresis. The single nucleotide polymorphism at the -521st position upstream of the initiation codon of the dopamine D4 receptor gene has two variations in the human population, a -521 C form and a -521 T form. Genotyping of -521 C/T single nucleotide polymorphism was performed by two independent methods - use of bidirectional allele-specific amplification, as described recently, and by restriction fragment site polymorphism using Fsp I enzyme. Only results giving the same genotypes by both methods were accepted. The genotyping methods were used to compare allele and genotype frequencies in 47 drug-user males and 118 controls. Our preliminary results showed a tendency for a higher frequency of the -521 C allele among drug users, but further investigations are needed to determine whether the results are statistically significant.

Original languageEnglish
Pages (from-to)101-106
Number of pages6
JournalJournal of Planar Chromatography - Modern TLC
Volume15
Issue number2
Publication statusPublished - 2002

Fingerprint

Electrophoresis
Polymorphism
Dopamine D4 Receptors
Single Nucleotide Polymorphism
Nucleotides
Drug Users
Amplification
Genes
Alleles
Genotype
Initiator Codon
Polymerase chain reaction
DNA sequences
Reaction products
Genetic Promoter Regions
Gene Frequency
Pharmaceutical Preparations
Gels
Sampling
Polymerase Chain Reaction

Keywords

  • Dopamine D4 receptor
  • Genotyping
  • Receptor gene polymorphism
  • Single-nucleotide polymorphism
  • Slab-gel electrophoresis

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

The use of planar electrophoresis for detection of single-nucleotide polymorphism. / Boór, Krisztina; Rónai, Z.; Sasvári, M.; Gaszner, Péter; Kalász, H.

In: Journal of Planar Chromatography - Modern TLC, Vol. 15, No. 2, 2002, p. 101-106.

Research output: Contribution to journalArticle

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