The use of molecular probes in the study of the action of histamine on macrophages

K. Merétey, A. Falus

Research output: Contribution to journalArticle

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Abstract

The effect of histamine on the expression in vitro of C2, factor B and C3 genes in murine resident peritoneal macrophages was investigated. By measuring the levels of specific mRNA and the amount of the biosynthetically labelled gene products, we found that histamine decreases the biosynthesis of C2 and factor B. However, histamine had virtually no effect on the biosynthesis of C3 in the same cells. To clarify the mechanism of these effects, the H1 agonists (2-pyridylethylamine (PEA)and 2-methylhistamine), the H2-agonists (impromidine and 4-methyl-histamine), the H1-antagonist (chlorpheniramine) as well as the H2-antagonist (cimetidine) were also tested. We found that the inhibitory effect of histamine on C2 and factor B is produced only through H2 receptors. With the combination of histamine agonists and antagonists an, inverse effect via H1-or H2-receptors was determined on the biosynthesis of C3. H1-receptor stimuli enhanced, and the stimulation of H2-receptors inhibited, C3 biosynthesis. All effects were pretranslational since the same results were obtained on mRNA and protein levels.

Original languageEnglish
Pages (from-to)328-330
Number of pages3
JournalAgents and Actions
Volume23
Issue number3-4
DOIs
Publication statusPublished - May 1988

Fingerprint

Molecular Probes
Macrophages
Biosynthesis
Histamine Agonists
Histamine
Histamine H2 Receptors
Histamine H1 Receptors
Impromidine
Genes
Histamine H1 Antagonists
Chlorpheniramine
Messenger RNA
Histamine Antagonists
Cimetidine
Peritoneal Macrophages
Proteins

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology (medical)

Cite this

The use of molecular probes in the study of the action of histamine on macrophages. / Merétey, K.; Falus, A.

In: Agents and Actions, Vol. 23, No. 3-4, 05.1988, p. 328-330.

Research output: Contribution to journalArticle

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