The use of microcarrier beads in the production of endothelium-derived relaxing factor by freshly harvested endothelial cells

R. J. Bing, T. Binder, J. Pataricza, S. Kibira, K. Shankar Narayan

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

This study is concerned with the use of freshly harvested bovine endothelial cells attached to microcarrier beads in the production of the endothelium-derived relaxing factor (EDRF). The results are compared to production of EDRF by endothelial cells grown in tissue cultures. We found that freshly harvested cells attach themselves to microcarrier beads within minutes. This results in large surface/area volume ratio and permits superfusion of cells suspension on a filter (pore size of 25-30 microns), resulting in cell free fitrate. When superfusing an endothelium-deprived pulmonary artery strip, the effluent causes relaxation; the response depends on the number of superfused endothelial cells. The number of viable freshly harvested cells attached to microcarrier beads in 5 ml Krebs-Henseleit solution is small (30%), as compared to almost 100% for cultured cells. Despite this difference, percent relaxation induced for the same number of viable cells is identical for both groups. Scanning electromicrographs confirm anchorage of endothelial cells to microcarrier beads. While cultured cells cover the entire surface and are individually attached, freshly harvested cells are anchored as cell aggregates leaving some of the surface free. Attachment of freshly harvested endothelial cells to microcarrier beads offers an alternative for the study of the role of endothelial cells in the production of vasoactive substances.

Original languageEnglish
Pages (from-to)151-159
Number of pages9
JournalTissue and Cell
Volume23
Issue number2
DOIs
Publication statusPublished - 1991

Fingerprint

Endothelium-Dependent Relaxing Factors
endothelium
endothelial cells
Endothelial Cells
cultured cells
cells
Cultured Cells
pulmonary artery
cell aggregates
cell suspension culture
Pulmonary Artery
Endothelium
tissue culture
effluents
surface area
Suspensions
Cell Count
cattle

Keywords

  • Endothelium
  • microcarrier beads
  • relaxing factor
  • vasoactive substances

ASJC Scopus subject areas

  • Insect Science
  • Cell Biology

Cite this

The use of microcarrier beads in the production of endothelium-derived relaxing factor by freshly harvested endothelial cells. / Bing, R. J.; Binder, T.; Pataricza, J.; Kibira, S.; Narayan, K. Shankar.

In: Tissue and Cell, Vol. 23, No. 2, 1991, p. 151-159.

Research output: Contribution to journalArticle

Bing, R. J. ; Binder, T. ; Pataricza, J. ; Kibira, S. ; Narayan, K. Shankar. / The use of microcarrier beads in the production of endothelium-derived relaxing factor by freshly harvested endothelial cells. In: Tissue and Cell. 1991 ; Vol. 23, No. 2. pp. 151-159.
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