The use of ionophores of rapid loading of human red cells with radioactive cations for cation-pump studies

B. Sarkadi, Ilma Szász, G. Gárdos

Research output: Contribution to journalArticle

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Abstract

Techniques are described for the rapid loading of intact human red cells with radioactive isotopes of alkali cations or Ca2+ by using ionophorous compounds (nigericin, gramicidin D and A 23187). Loading was rapid and efficient if the membrane potential of the cells was rendered more negative inside. After cation loading the ionophores could be bound to albumin and removed by repeated washings. The ATP and 2,3-DPG contents of the cells were practically unaltered by this treatment. Passive membrane permeability to Na+ and Ca2+ returned to normal. Loaded erythrocytes pumped out Na+ in a ouabain-sensitive and Ca2+ in a lanthanum-sensitive way. Ca2+-loaded red cells were microspherocytes and exhibited a rapid K+-efflux. Parallel with the extrusion of Ca2+ cells regained their biconcave shape and normal passive permeability to K+.

Original languageEnglish
Pages (from-to)357-370
Number of pages14
JournalThe Journal of Membrane Biology
Volume26
Issue number1
DOIs
Publication statusPublished - Dec 1976

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Cation Transport Proteins
Ionophores
Cations
Gramicidin
Permeability
Nigericin
2,3-Diphosphoglycerate
Lanthanum
Alkalies
Ouabain
Radioisotopes
Membrane Potentials
Albumins
Adenosine Triphosphate
Erythrocytes
Membranes

ASJC Scopus subject areas

  • Physiology
  • Cell Biology
  • Biophysics

Cite this

The use of ionophores of rapid loading of human red cells with radioactive cations for cation-pump studies. / Sarkadi, B.; Szász, Ilma; Gárdos, G.

In: The Journal of Membrane Biology, Vol. 26, No. 1, 12.1976, p. 357-370.

Research output: Contribution to journalArticle

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