The use of antigen microarrays in antibody profiling

Krisztián Papp, József Prechl

Research output: Chapter in Book/Report/Conference proceedingChapter

9 Citations (Scopus)

Abstract

Technological advances in the field of microarray production and analysis lead to the development of protein microarrays. Of these, antigen microarrays are one particular format that allows the study of antigen-antibody interactions in a miniaturized and highly multiplexed fashion. Here, we describe the parallel detection of antibodies with different specificities in human serum, a procedure also called antibody profiling. Autoantigens printed on microarray slides are reacted with test sera and the bound antibodies are identified by fluorescently labeled secondary reagents. Reactivity patterns generated this way characterize individuals and can help design novel diagnostic tools.

Original languageEnglish
Title of host publicationFunctional Genomics
Subtitle of host publicationMethods and Protocols
EditorsMichael Kaufmann, Claudia Klinger
Pages175-185
Number of pages11
DOIs
Publication statusPublished - Jan 2 2012

Publication series

NameMethods in Molecular Biology
Volume815
ISSN (Print)1064-3745

Keywords

  • Antibody
  • Antigen
  • Immunity
  • Immunoassay
  • Immunoglobulin
  • Profiling
  • Protein microarray

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Papp, K., & Prechl, J. (2012). The use of antigen microarrays in antibody profiling. In M. Kaufmann, & C. Klinger (Eds.), Functional Genomics: Methods and Protocols (pp. 175-185). (Methods in Molecular Biology; Vol. 815). https://doi.org/10.1007/978-1-61779-424-7_14