The use of a sodium tungstate developer markedly improves the electron microscopic localization of zinc by the Timm method

L. Séress, F. erenc Gallyas

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The Timm's sulfide-silver method is frequently used for the demonstration of the mossy fiber bundle or sprouted mossy fibers in the normal or epileptic hippocampal dentate gyrus. Under the light microscope the results are excellent, but the ultrastructure is considerably impaired and the silver grains produced are too large as compared to the sizes of intra-synaptic structures. The present study was meant to test a series of physical developers containing, instead of gum arabic, sodium tungstate as protective colloid. One of them left the ultrastructure fairly intact and produced small, round silver grains, making it possible to precisely locate zinc in mossy terminals. With this method, it could be demonstrated that zinc is contained inside synaptic vesicles in the resting axon terminals of granule cells. As a consequence of prolonged sodium sulfide perfusion, zinc is released from synaptic vesicles and enters the synaptic cleft. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)33-39
Number of pages7
JournalJournal of Neuroscience Methods
Volume100
Issue number1-2
DOIs
Publication statusPublished - Jul 31 2000

Fingerprint

Zinc
Synaptic Vesicles
Electrons
Silver
Gum Arabic
Parahippocampal Gyrus
Dentate Gyrus
Presynaptic Terminals
Colloids
Perfusion
Light
sodium tungstate(VI)
sodium sulfide
silver sulfide

Keywords

  • Hippocampal dentate gyrus
  • Mossy fiber terminals
  • Silver plating
  • Tungstate physical developer
  • Ultrastructural location of zinc

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

The use of a sodium tungstate developer markedly improves the electron microscopic localization of zinc by the Timm method. / Séress, L.; Gallyas, F. erenc.

In: Journal of Neuroscience Methods, Vol. 100, No. 1-2, 31.07.2000, p. 33-39.

Research output: Contribution to journalArticle

@article{ae7b7991130b4ecab3b6731a246ace5b,
title = "The use of a sodium tungstate developer markedly improves the electron microscopic localization of zinc by the Timm method",
abstract = "The Timm's sulfide-silver method is frequently used for the demonstration of the mossy fiber bundle or sprouted mossy fibers in the normal or epileptic hippocampal dentate gyrus. Under the light microscope the results are excellent, but the ultrastructure is considerably impaired and the silver grains produced are too large as compared to the sizes of intra-synaptic structures. The present study was meant to test a series of physical developers containing, instead of gum arabic, sodium tungstate as protective colloid. One of them left the ultrastructure fairly intact and produced small, round silver grains, making it possible to precisely locate zinc in mossy terminals. With this method, it could be demonstrated that zinc is contained inside synaptic vesicles in the resting axon terminals of granule cells. As a consequence of prolonged sodium sulfide perfusion, zinc is released from synaptic vesicles and enters the synaptic cleft. (C) 2000 Elsevier Science B.V.",
keywords = "Hippocampal dentate gyrus, Mossy fiber terminals, Silver plating, Tungstate physical developer, Ultrastructural location of zinc",
author = "L. S{\'e}ress and Gallyas, {F. erenc}",
year = "2000",
month = "7",
day = "31",
doi = "10.1016/S0165-0270(00)00227-2",
language = "English",
volume = "100",
pages = "33--39",
journal = "Journal of Neuroscience Methods",
issn = "0165-0270",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - The use of a sodium tungstate developer markedly improves the electron microscopic localization of zinc by the Timm method

AU - Séress, L.

AU - Gallyas, F. erenc

PY - 2000/7/31

Y1 - 2000/7/31

N2 - The Timm's sulfide-silver method is frequently used for the demonstration of the mossy fiber bundle or sprouted mossy fibers in the normal or epileptic hippocampal dentate gyrus. Under the light microscope the results are excellent, but the ultrastructure is considerably impaired and the silver grains produced are too large as compared to the sizes of intra-synaptic structures. The present study was meant to test a series of physical developers containing, instead of gum arabic, sodium tungstate as protective colloid. One of them left the ultrastructure fairly intact and produced small, round silver grains, making it possible to precisely locate zinc in mossy terminals. With this method, it could be demonstrated that zinc is contained inside synaptic vesicles in the resting axon terminals of granule cells. As a consequence of prolonged sodium sulfide perfusion, zinc is released from synaptic vesicles and enters the synaptic cleft. (C) 2000 Elsevier Science B.V.

AB - The Timm's sulfide-silver method is frequently used for the demonstration of the mossy fiber bundle or sprouted mossy fibers in the normal or epileptic hippocampal dentate gyrus. Under the light microscope the results are excellent, but the ultrastructure is considerably impaired and the silver grains produced are too large as compared to the sizes of intra-synaptic structures. The present study was meant to test a series of physical developers containing, instead of gum arabic, sodium tungstate as protective colloid. One of them left the ultrastructure fairly intact and produced small, round silver grains, making it possible to precisely locate zinc in mossy terminals. With this method, it could be demonstrated that zinc is contained inside synaptic vesicles in the resting axon terminals of granule cells. As a consequence of prolonged sodium sulfide perfusion, zinc is released from synaptic vesicles and enters the synaptic cleft. (C) 2000 Elsevier Science B.V.

KW - Hippocampal dentate gyrus

KW - Mossy fiber terminals

KW - Silver plating

KW - Tungstate physical developer

KW - Ultrastructural location of zinc

UR - http://www.scopus.com/inward/record.url?scp=0034738329&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034738329&partnerID=8YFLogxK

U2 - 10.1016/S0165-0270(00)00227-2

DO - 10.1016/S0165-0270(00)00227-2

M3 - Article

C2 - 11040364

AN - SCOPUS:0034738329

VL - 100

SP - 33

EP - 39

JO - Journal of Neuroscience Methods

JF - Journal of Neuroscience Methods

SN - 0165-0270

IS - 1-2

ER -