The use of a flagellar export signal for the secretion of recombinant proteins in Salmonella

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

The flagellum-specific export system is a specialized type III export machinery, which exports external flagellar proteins through the central channel of the flagellar filament. A number of evidence indicates that short segments within the disordered N-terminal region of flagellar axial proteins are recognized by the flagellum-specific export apparatus. Recently, we have demonstrated that the 26-47 segment of Salmonella typhimurium flagellin is capable of mediating flagellar export. N-terminal flagellin segments containing the export signal combined with a hexahistidine tag can be attached to heterologous proteins (preferentially in the size range of 9-40 kDa) facilitating their secreted expression and easy purification from the medium. Certain over-expressed proteins that are easily degraded within the cells are found intact in the medium implying a potential application of this expression system for proteins of high proteolytic susceptibility.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages131-143
Number of pages13
Volume824
DOIs
Publication statusPublished - 2012

Publication series

NameMethods in Molecular Biology
Volume824
ISSN (Print)10643745

Fingerprint

Recombinant Proteins
Salmonella
Flagellin
His-His-His-His-His-His
Flagella
Proteins
Salmonella typhimurium

Keywords

  • Export signal
  • Flagellin
  • Flagellum-specific export
  • Recombinant protein
  • Salmonella typhimurium
  • Secreted over-expression
  • Type III secretion

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Vonderviszt, F., Sajó, R., Dobó, J., & Závodszky, P. (2012). The use of a flagellar export signal for the secretion of recombinant proteins in Salmonella. In Methods in Molecular Biology (Vol. 824, pp. 131-143). (Methods in Molecular Biology; Vol. 824). https://doi.org/10.1007/978-1-61779-433-9_6

The use of a flagellar export signal for the secretion of recombinant proteins in Salmonella. / Vonderviszt, F.; Sajó, Ráchel; Dobó, J.; Závodszky, P.

Methods in Molecular Biology. Vol. 824 2012. p. 131-143 (Methods in Molecular Biology; Vol. 824).

Research output: Chapter in Book/Report/Conference proceedingChapter

Vonderviszt, F. ; Sajó, Ráchel ; Dobó, J. ; Závodszky, P. / The use of a flagellar export signal for the secretion of recombinant proteins in Salmonella. Methods in Molecular Biology. Vol. 824 2012. pp. 131-143 (Methods in Molecular Biology).
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