The uptake of biogenic monoamines by the isolated auricle of the snail (Helix pomatia)

L. Hiripi, V. Neuhoff, N. N. Osborne

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The uptake of [3H]5HT, [3H]dopamine, [3H]noradrenaline and [3H]octopamine into the auricle of Helix pomatia was studied. When tissues were incubated at 25°C in media containing radioactive amines, tissue:medium ratios of about 49:1, 14:1 and 5:1 for 5 HT, dopamine, noradrenaline, and octopamine respectively were obtained after a 20-30 min incubation time. Tissues incubated at 25°C in media containing radioactive amines for 20-30 mins showed that almost all (96%) the radioactivity was present as unchanged [3H]5 HT, [3H]dopamine, [3H]octopamine or [3H]noradrenaline. The high tissue:medium ratios for 5 HT and dopamine, but not for noradrenaline and octopamine, showed saturation kinetics which were dependent upon temperature and sodium ions. From the Lineweaver Burk plots, two uptake mechanisms for 5 HT at 25°C were resolved; the high affinity uptake process having a K(m1) value of 6.0 x 10-8 M and a V(m1) value of 0.115 nmol/g/min while the lower affinity process had a K(m2) value of 1.04 x 10-6 M and a V(m2) value of 0.66 nmol/g/min. At 0°C a single uptake mechanism for 5 HT occurred which gave a K(m) value of 5.02 x 10-8 M and a V(m) value of 0.0165 nmol/g/min. In the case of dopamine, the Lineweaver Burk plot at 25°C showed a single uptake process with values for K(m) and V(m) of 1.55 x 10-7 M and 0.086 nmol/g/min respectively. This process did not function at 0°C. The effect of various agents and ions upon the accumulation processes for all amines was also studied, and the data indicate that the same neurons probably accumulate more than one amine type. It is concluded that 5 HT and dopamine uptake in the auricle is a mechanism for inactivating these substances at 25°C and that an uptake mechanism for 5 HT also functions at 0°C. The results are discussed from the point of view of 5 HT's being the cardioexcitatory substance in the snail heart.

Original languageEnglish
Pages (from-to)335-343
Number of pages9
JournalJournal of Neurochemistry
Volume26
Issue number2
Publication statusPublished - 1976

Fingerprint

Biogenic Monoamines
Helix (Snails)
Serotonin
Octopamine
Dopamine
Amines
Norepinephrine
Tissue
Ions
Snails
Radioactivity
Neurons
Sodium

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

The uptake of biogenic monoamines by the isolated auricle of the snail (Helix pomatia). / Hiripi, L.; Neuhoff, V.; Osborne, N. N.

In: Journal of Neurochemistry, Vol. 26, No. 2, 1976, p. 335-343.

Research output: Contribution to journalArticle

@article{b64569a324404ae08d43471ad7fbc213,
title = "The uptake of biogenic monoamines by the isolated auricle of the snail (Helix pomatia)",
abstract = "The uptake of [3H]5HT, [3H]dopamine, [3H]noradrenaline and [3H]octopamine into the auricle of Helix pomatia was studied. When tissues were incubated at 25°C in media containing radioactive amines, tissue:medium ratios of about 49:1, 14:1 and 5:1 for 5 HT, dopamine, noradrenaline, and octopamine respectively were obtained after a 20-30 min incubation time. Tissues incubated at 25°C in media containing radioactive amines for 20-30 mins showed that almost all (96{\%}) the radioactivity was present as unchanged [3H]5 HT, [3H]dopamine, [3H]octopamine or [3H]noradrenaline. The high tissue:medium ratios for 5 HT and dopamine, but not for noradrenaline and octopamine, showed saturation kinetics which were dependent upon temperature and sodium ions. From the Lineweaver Burk plots, two uptake mechanisms for 5 HT at 25°C were resolved; the high affinity uptake process having a K(m1) value of 6.0 x 10-8 M and a V(m1) value of 0.115 nmol/g/min while the lower affinity process had a K(m2) value of 1.04 x 10-6 M and a V(m2) value of 0.66 nmol/g/min. At 0°C a single uptake mechanism for 5 HT occurred which gave a K(m) value of 5.02 x 10-8 M and a V(m) value of 0.0165 nmol/g/min. In the case of dopamine, the Lineweaver Burk plot at 25°C showed a single uptake process with values for K(m) and V(m) of 1.55 x 10-7 M and 0.086 nmol/g/min respectively. This process did not function at 0°C. The effect of various agents and ions upon the accumulation processes for all amines was also studied, and the data indicate that the same neurons probably accumulate more than one amine type. It is concluded that 5 HT and dopamine uptake in the auricle is a mechanism for inactivating these substances at 25°C and that an uptake mechanism for 5 HT also functions at 0°C. The results are discussed from the point of view of 5 HT's being the cardioexcitatory substance in the snail heart.",
author = "L. Hiripi and V. Neuhoff and Osborne, {N. N.}",
year = "1976",
language = "English",
volume = "26",
pages = "335--343",
journal = "Journal of Neurochemistry",
issn = "0022-3042",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - The uptake of biogenic monoamines by the isolated auricle of the snail (Helix pomatia)

AU - Hiripi, L.

AU - Neuhoff, V.

AU - Osborne, N. N.

PY - 1976

Y1 - 1976

N2 - The uptake of [3H]5HT, [3H]dopamine, [3H]noradrenaline and [3H]octopamine into the auricle of Helix pomatia was studied. When tissues were incubated at 25°C in media containing radioactive amines, tissue:medium ratios of about 49:1, 14:1 and 5:1 for 5 HT, dopamine, noradrenaline, and octopamine respectively were obtained after a 20-30 min incubation time. Tissues incubated at 25°C in media containing radioactive amines for 20-30 mins showed that almost all (96%) the radioactivity was present as unchanged [3H]5 HT, [3H]dopamine, [3H]octopamine or [3H]noradrenaline. The high tissue:medium ratios for 5 HT and dopamine, but not for noradrenaline and octopamine, showed saturation kinetics which were dependent upon temperature and sodium ions. From the Lineweaver Burk plots, two uptake mechanisms for 5 HT at 25°C were resolved; the high affinity uptake process having a K(m1) value of 6.0 x 10-8 M and a V(m1) value of 0.115 nmol/g/min while the lower affinity process had a K(m2) value of 1.04 x 10-6 M and a V(m2) value of 0.66 nmol/g/min. At 0°C a single uptake mechanism for 5 HT occurred which gave a K(m) value of 5.02 x 10-8 M and a V(m) value of 0.0165 nmol/g/min. In the case of dopamine, the Lineweaver Burk plot at 25°C showed a single uptake process with values for K(m) and V(m) of 1.55 x 10-7 M and 0.086 nmol/g/min respectively. This process did not function at 0°C. The effect of various agents and ions upon the accumulation processes for all amines was also studied, and the data indicate that the same neurons probably accumulate more than one amine type. It is concluded that 5 HT and dopamine uptake in the auricle is a mechanism for inactivating these substances at 25°C and that an uptake mechanism for 5 HT also functions at 0°C. The results are discussed from the point of view of 5 HT's being the cardioexcitatory substance in the snail heart.

AB - The uptake of [3H]5HT, [3H]dopamine, [3H]noradrenaline and [3H]octopamine into the auricle of Helix pomatia was studied. When tissues were incubated at 25°C in media containing radioactive amines, tissue:medium ratios of about 49:1, 14:1 and 5:1 for 5 HT, dopamine, noradrenaline, and octopamine respectively were obtained after a 20-30 min incubation time. Tissues incubated at 25°C in media containing radioactive amines for 20-30 mins showed that almost all (96%) the radioactivity was present as unchanged [3H]5 HT, [3H]dopamine, [3H]octopamine or [3H]noradrenaline. The high tissue:medium ratios for 5 HT and dopamine, but not for noradrenaline and octopamine, showed saturation kinetics which were dependent upon temperature and sodium ions. From the Lineweaver Burk plots, two uptake mechanisms for 5 HT at 25°C were resolved; the high affinity uptake process having a K(m1) value of 6.0 x 10-8 M and a V(m1) value of 0.115 nmol/g/min while the lower affinity process had a K(m2) value of 1.04 x 10-6 M and a V(m2) value of 0.66 nmol/g/min. At 0°C a single uptake mechanism for 5 HT occurred which gave a K(m) value of 5.02 x 10-8 M and a V(m) value of 0.0165 nmol/g/min. In the case of dopamine, the Lineweaver Burk plot at 25°C showed a single uptake process with values for K(m) and V(m) of 1.55 x 10-7 M and 0.086 nmol/g/min respectively. This process did not function at 0°C. The effect of various agents and ions upon the accumulation processes for all amines was also studied, and the data indicate that the same neurons probably accumulate more than one amine type. It is concluded that 5 HT and dopamine uptake in the auricle is a mechanism for inactivating these substances at 25°C and that an uptake mechanism for 5 HT also functions at 0°C. The results are discussed from the point of view of 5 HT's being the cardioexcitatory substance in the snail heart.

UR - http://www.scopus.com/inward/record.url?scp=0017253595&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017253595&partnerID=8YFLogxK

M3 - Article

C2 - 768414

AN - SCOPUS:0017253595

VL - 26

SP - 335

EP - 343

JO - Journal of Neurochemistry

JF - Journal of Neurochemistry

SN - 0022-3042

IS - 2

ER -