The ultrastructure and flexibility of thylakoid membranes in leaves and isolated chloroplasts as revealed by small-angle neutron scattering

R. Ünnep, O. Zsiros, K. Solymosi, L. Kovács, P. Lambrev, T. Tóth, R. Schweins, D. Posselt, N. K. Székely, L. Rosta, G. Nagy, G. Garab

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

We studied the periodicity of the multilamellar membrane system of granal chloroplasts in different isolated plant thylakoid membranes, using different suspension media, as well as on different detached leaves and isolated protoplasts - using small-angle neutron scattering. Freshly isolated thylakoid membranes suspended in isotonic or hypertonic media, containing sorbitol supplemented with cations, displayed Bragg peaks typically between 0.019 and 0.023 Å- 1, corresponding to spatially and statistically averaged repeat distance values of about 275-330 Å. Similar data obtained earlier led us in previous work to propose an origin from the periodicity of stroma thylakoid membranes. However, detached leaves, of eleven different species, infiltrated with or soaked in D2O in dim laboratory light or transpired with D2O prior to measurements, exhibited considerably smaller repeat distances, typically between 210 and 230 Å, ruling out a stromal membrane origin. Similar values were obtained on isolated tobacco and spinach protoplasts. When NaCl was used as osmoticum, the Bragg peaks of isolated thylakoid membranes almost coincided with those in the same batch of leaves and the repeat distances were very close to the electron microscopically determined values in the grana. Although neutron scattering and electron microscopy yield somewhat different values, which is not fully understood, we can conclude that small-angle neutron scattering is a suitable technique to study the periodic organization of granal thylakoid membranes in intact leaves under physiological conditions and with a time resolution of minutes or shorter. We also show here, for the first time on leaves, that the periodicity of thylakoid membranes in situ responds dynamically to moderately strong illumination. This article is part of a Special Issue entitled: Photosynthesis research for sustainability: Keys to produce clean energy.

Original languageEnglish
Pages (from-to)1572-1580
Number of pages9
JournalBBA - Bioenergetics
Volume1837
Issue number9
DOIs
Publication statusPublished - 2014

Fingerprint

Small Angle Scattering
Thylakoids
Neutrons
Chloroplasts
Neutron scattering
Membranes
Periodicity
Protoplasts
Spinacia oleracea
Sorbitol
Photosynthesis
Tobacco
Lighting
Cations
Suspensions
Electron Microscopy
Electron microscopy
Sustainable development
Electrons
Light

Keywords

  • Electron microscopy
  • Granum
  • Lamellar repeat distance
  • Small-angle neutron scattering
  • Thylakoid membrane

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology

Cite this

The ultrastructure and flexibility of thylakoid membranes in leaves and isolated chloroplasts as revealed by small-angle neutron scattering. / Ünnep, R.; Zsiros, O.; Solymosi, K.; Kovács, L.; Lambrev, P.; Tóth, T.; Schweins, R.; Posselt, D.; Székely, N. K.; Rosta, L.; Nagy, G.; Garab, G.

In: BBA - Bioenergetics, Vol. 1837, No. 9, 2014, p. 1572-1580.

Research output: Contribution to journalArticle

Ünnep, R. ; Zsiros, O. ; Solymosi, K. ; Kovács, L. ; Lambrev, P. ; Tóth, T. ; Schweins, R. ; Posselt, D. ; Székely, N. K. ; Rosta, L. ; Nagy, G. ; Garab, G. / The ultrastructure and flexibility of thylakoid membranes in leaves and isolated chloroplasts as revealed by small-angle neutron scattering. In: BBA - Bioenergetics. 2014 ; Vol. 1837, No. 9. pp. 1572-1580.
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AU - Kovács, L.

AU - Lambrev, P.

AU - Tóth, T.

AU - Schweins, R.

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