The tumor promoter tetradecanoylphorbol-13-acetate elicits the redistribution of heavy metals in subcellular fractions of rabbit thymocytes as measured by plasma emission spectroscopy

Péter Csermely, Péter Fodor, János Somogyi

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Abstract

Prolonged (90 min) incubation with the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) causes no significant changes in the elemental composition of whole rabbit thymocytes (25 elements included). The only exception is the amount of magnesium, where a significant increase can be detected. However TPA preincubation elicits the redistribution of heavy metals (mainly Zn and to a much lesser extent Fe and Cu) from the nuclear and large granular (mitochondrial-lysosomal) fraction to the cytosol and microsomes. A significant translocation of calcium and phosphorus from the large granular fraction mainly to the nucleus can also be observed. The TPA-induced heavy metal depletion of the nucleus might play a role in the antiproliferative activity of TPA, the arrest of G1→S phase transition. On the other hand the increased amount of heavy metals in the cytosol might be linked to the TPA-induced block of intercellular communication and the desensitization of TPA-treated cells towards extracellular calcium which are well-known properties of neoplastic cells.

Original languageEnglish
Pages (from-to)1663-1666
Number of pages4
JournalCarcinogenesis
Volume8
Issue number11
DOIs
Publication statusPublished - Nov 1 1987

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ASJC Scopus subject areas

  • Cancer Research

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