The trafficking pathway of a wheat storage protein in transgenic rice endosperm

Maria Oszvald, L. Tamás, Peter R. Shewry, Paola Tosi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background and AimsThe trafficking of proteins in the endoplasmic reticulum (ER) of plant cells is a topic of considerable interest since this organelle serves as an entry point for proteins destined for other organelles, as well as for the ER itself. In the current work, transgenic rice was used to study the pattern and pathway of deposition of the wheat high molecular weight (HMW) glutenin sub-unit (GS) 1Dx5 within the rice endosperm using specific antibodies to determine whether it is deposited in the same or different protein bodies from the rice storage proteins, and whether it is located in the same or separate phases within these.MethodsThe protein distribution and the expression pattern of HMW sub-unit 1Dx5 in transgenic rice endosperm at different stages of development were determined using light and electron microscopy after labelling with antibodies.Key resultsThe use of HMW-GS-specific antibodies showed that sub-unit 1Dx5 was expressed mainly in the sub-aleurone cells of the endosperm and that it was deposited in both types of protein body present in the rice endosperm: derived from the ER and containing prolamins, and derived from the vacuole and containing glutelins. In addition, new types of protein bodies were also formed within the endosperm cells.ConclusionsThe results suggest that the HMW 1Dx5 protein could be trafficked by either the ER or vacuolar pathway, possibly depending on the stage of development, and that its accumulation in the rice endosperm could compromise the structural integrity of protein bodies and their segregation into two distinct populations in the mature endosperm.

Original languageEnglish
Pages (from-to)807-815
Number of pages9
JournalAnnals of Botany
Volume113
Issue number5
DOIs
Publication statusPublished - 2014

Fingerprint

Endosperm
wheat protein
storage proteins
Triticum
endosperm
genetically modified organisms
protein bodies
rice
endoplasmic reticulum
Endoplasmic Reticulum
Proteins
molecular weight
Molecular Weight
Somatotypes
glutenins
antibodies
organelles
Organelles
Antibodies
aleurone cells

Keywords

  • cereal endosperm
  • HMW-GS
  • immunolocalization
  • Oryza sativa
  • protein bodies
  • protein trafficking
  • transgenic rice
  • Triticum aestivum
  • wheat

ASJC Scopus subject areas

  • Plant Science
  • Medicine(all)

Cite this

The trafficking pathway of a wheat storage protein in transgenic rice endosperm. / Oszvald, Maria; Tamás, L.; Shewry, Peter R.; Tosi, Paola.

In: Annals of Botany, Vol. 113, No. 5, 2014, p. 807-815.

Research output: Contribution to journalArticle

Oszvald, Maria ; Tamás, L. ; Shewry, Peter R. ; Tosi, Paola. / The trafficking pathway of a wheat storage protein in transgenic rice endosperm. In: Annals of Botany. 2014 ; Vol. 113, No. 5. pp. 807-815.
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AB - Background and AimsThe trafficking of proteins in the endoplasmic reticulum (ER) of plant cells is a topic of considerable interest since this organelle serves as an entry point for proteins destined for other organelles, as well as for the ER itself. In the current work, transgenic rice was used to study the pattern and pathway of deposition of the wheat high molecular weight (HMW) glutenin sub-unit (GS) 1Dx5 within the rice endosperm using specific antibodies to determine whether it is deposited in the same or different protein bodies from the rice storage proteins, and whether it is located in the same or separate phases within these.MethodsThe protein distribution and the expression pattern of HMW sub-unit 1Dx5 in transgenic rice endosperm at different stages of development were determined using light and electron microscopy after labelling with antibodies.Key resultsThe use of HMW-GS-specific antibodies showed that sub-unit 1Dx5 was expressed mainly in the sub-aleurone cells of the endosperm and that it was deposited in both types of protein body present in the rice endosperm: derived from the ER and containing prolamins, and derived from the vacuole and containing glutelins. In addition, new types of protein bodies were also formed within the endosperm cells.ConclusionsThe results suggest that the HMW 1Dx5 protein could be trafficked by either the ER or vacuolar pathway, possibly depending on the stage of development, and that its accumulation in the rice endosperm could compromise the structural integrity of protein bodies and their segregation into two distinct populations in the mature endosperm.

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