The tissue-specific expression of a tobacco phytochrome B gene

Eva Adam, Laszlo Kozma-Bognar, Claudia Kolar, Eberhard Schafer, Ferenc Nagy

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We have isolated a genomic clone from Nicotiana tabacum, designated Nt-PHYB-1, encoding a type-II, "green tissue" phytochrome apoprotein. Recombinant genes, consisting of the 3319-bp promoter of the Nt-PHYB-1 gene (including the entire 5′ untranslated sequence but not the ATG) or its deletion derivatives and the bacterial β-glucuronidase reporter gene, were constructed and transferred into tobacco. The expression patterns and levels of the endogenous Nt-PHYB-1, as well as those of the transgenes, were determined by RNase protection assays and by β-glucuronidase histochemical staining. We show that (a) the PHYB-1 gene has three transcription start sites, (b) the abundance of the three PHYB-1-specific mRNAs is different, and that (c) it is not regulated by light. However, we do demonstrate that transcription of the endogenous PHYB-1 gene and that of the recombinant genes exhibit a well-defined organ and tissue specificity. This tobacco PHYB gene is relatively highly expressed in leaf, stem, and different floral organs but not in root. Deletion analysis of the Nt-PHYB-1 promoter indicates that a 382-bp region, located between -1472 and -1089, is required for high-level expression of this gene.

Original languageEnglish
Pages (from-to)1081-1088
Number of pages8
JournalPlant physiology
Issue number4
Publication statusPublished - Apr 1996


ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

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