The three-dimensional network of the thylakoid membranes in plants

Quasihelical model of the granum-stroma assembly

L. Mustárdy, Karolyn Buttle, Gábor Steinbach, G. Garab

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

The three-dimensional (3-D) network of the granum-stroma thylakoid assembly of vascular plant chloroplasts exhibits complex structural/functional heterogeneity. A complete understanding of the ultrastructure of this assembly is critical for our understanding of thylakoid function. The prevailing historical model of thylakoid structure, based on information derived from serial section analyses of electron microscopy (EM) images, suggests a helical arrangement of stroma membranes wound around the granum stacks. More recently, electron tomography has emerged as the leading method for the study of thylakoid ultrastructure, as it provides for higher resolution in the depth dimension. The first detailed 3D topological model derived from electron tomography was in disagreement with the helical model, whereas a more recent electron tomography study, conducted under somewhat different experimental conditions, suggested that basic features of the helical model are still valid. Here, we review the conventional EM data and present a critical discussion of the two electron tomography data sets in an attempt to establish a consensus model that accommodates all the information presently available.

Original languageEnglish
Pages (from-to)2552-2557
Number of pages6
JournalPlant Cell
Volume20
Issue number10
DOIs
Publication statusPublished - Oct 2008

Fingerprint

grana
Thylakoids
thylakoids
Electron Microscope Tomography
tomography
electrons
ultrastructure
electron microscopy
Electron Microscopy
plant damage
vascular plants
Chloroplasts
chloroplasts
Blood Vessels
Membranes
Wounds and Injuries

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

The three-dimensional network of the thylakoid membranes in plants : Quasihelical model of the granum-stroma assembly. / Mustárdy, L.; Buttle, Karolyn; Steinbach, Gábor; Garab, G.

In: Plant Cell, Vol. 20, No. 10, 10.2008, p. 2552-2557.

Research output: Contribution to journalArticle

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