The membrane impairing effects of free radicals generated by phenazine methosulfate (PMS) and quinhydrone, resp. were studied on in vitro erythrocyte models with special regard to the particular processes in erythrocytes in case of lead pollution. Conclusions on the degree of membrane impairment were based on the extent of the intracellular K+ ion outflow measured at various dates. It has been found that the membrane impairing effect increases on the action of endogenous reduction (NADH) and in presence of lead, as compared to the membrane impairing effects of PMS and quinhydrone generated superoxide radicals. Based on these experiences the degree of the membrane impairment can be reduced by high energy phosphate compounds (ATP, ADP, AMP) in dependence of their energy content. It is being assumed that the outstanding toxicity of lead might be related to the Pb2+/Pb4+ superoxide radicals induced transformation.
|Number of pages||4|
|Publication status||Published - Jan 1 1995|
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