The stimulatory effect of salsolinol on prolactin gene expression within the anterior pituitary of lactating sheep: In vivo and in vitro study

Małgorzata Hasiec, Andrzej P. Herman, Edyta Molik, Elzbieta Dobek, Katarzyna Romanowicz, F. Fülöp, Tomasz Misztal

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Salsolinol (a derivative of dopamine) is involved in the stimulatory mechanism of pituitary prolactin (PRL) release in lactating sheep. In the current study, we examined a hypothesis that salsolinol could also affect the expression of the PRL gene in the ovine anterior pituitary (AP). Two experiments, in vivo and in vitro, were performed 48. h after lambs weaning in order to verify this hypothesis on sheep. In the in vivo experiment, sheep were infused intracerebroventriculary with salsolinol (total 150 μg) or a vehicle (control) in a series of five 30-min infusions at 30-min intervals. In the in vitro experiment, the explants of the AP tissue from another group of lactating sheep were incubated in a medium containing of 20 or 100 μg/ml of salsolinol for two 30-min periods with 15-min interval or in a pure medium (control). In the course of these experiments, blood or medium samples were collected every 10 or 15. min., respectively, to measure the prolactin concentration. The PRL mRNA expression was determined in the AP by real-time PCR. Salsolinol significantly increased the amount of PRL mRNA in the AP, compared to the control (P<0.01 for in vivo; P<0.01 and P<0.001 for lower and higher in vitro dose, respectively). The concentrations of PRL in plasma and medium were also significantly higher in salsolinol-treated groups, compared to controls (P<0.001 and P<0.05 for in vivo and higher in vitro dose, respectively). In conclusion, salsolinol plays a pivotal role in the regulation of both the pituitary PRL synthesis and release in lactating sheep.

Original languageEnglish
Pages (from-to)202-207
Number of pages6
JournalSmall Ruminant Research
Volume102
Issue number2-3
DOIs
Publication statusPublished - Feb 2012

Fingerprint

anterior pituitary
prolactin
in vitro studies
Prolactin
Sheep
Gene Expression
sheep
gene expression
Messenger RNA
dosage
In Vitro Techniques
salsolinol
Weaning
dopamine
Real-Time Polymerase Chain Reaction
Dopamine
weaning
explants
quantitative polymerase chain reaction
lambs

Keywords

  • Anterior pituitary
  • Gene expression
  • Lactation
  • Prolactin
  • Salsolinol

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Food Animals

Cite this

The stimulatory effect of salsolinol on prolactin gene expression within the anterior pituitary of lactating sheep : In vivo and in vitro study. / Hasiec, Małgorzata; Herman, Andrzej P.; Molik, Edyta; Dobek, Elzbieta; Romanowicz, Katarzyna; Fülöp, F.; Misztal, Tomasz.

In: Small Ruminant Research, Vol. 102, No. 2-3, 02.2012, p. 202-207.

Research output: Contribution to journalArticle

Hasiec, Małgorzata ; Herman, Andrzej P. ; Molik, Edyta ; Dobek, Elzbieta ; Romanowicz, Katarzyna ; Fülöp, F. ; Misztal, Tomasz. / The stimulatory effect of salsolinol on prolactin gene expression within the anterior pituitary of lactating sheep : In vivo and in vitro study. In: Small Ruminant Research. 2012 ; Vol. 102, No. 2-3. pp. 202-207.
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