The stearoyl-coenzyme a desaturase 1 is essential for virulence and membrane stress in candida parapsilosis through unsaturated fatty acid production

Long NamNguyen, A. Gácser, Joshua D. Nosanchuk

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Unsaturated fatty acids (UFA) are essential components of cells. In Saccharomyces cerevisiae, stearoylcoenzyme A (CoA) desaturase 1 (OLE1) affects cell viability through the regulation of oleic (18:1) or palmitoleic (16:1) acid production. In this study, we used a targeted gene deletion approach to determine the impact of OLE1 on the emerging human pathogenic fungus Candida parapsilosis. We found that the deletion of OLE1 resulted in an auxotrophic yeast strain (designated OLE1 KO) that required unsaturated fatty acids for growth but not saturated fatty acids. Additionally, the production of UFA by OLE1 KO yeast cells was markedly reduced, suggesting that Ole1 is essential for UFA production. In contrast to wild-type C. parapsilosis, which produced pseudohyphal growth on UFA-supplemented medium agar, pseudohyphal formation in the OLE1 KO cells was severely impaired, suggesting that Ole1 regulates morphology. Furthermore, the OLE1 KO cells were hypersensitive to various stress-inducing factors, such as salts, SDS, and H2O2, especially at the physiological temperature. The results indicate that OLE1 is essential for the stress response, perhaps through the production of UFA for cell membrane biosynthesis. The OLE1 KO cells also were hypersensitive to human and fetal bovine serum, suggesting that targeting Ole1 could suppress the dissemination of yeast cells in the bloodstream. Murine-like macrophage J774.16 more efficiently killed the OLE1 KO yeasts, and significantly larger amounts of nitric oxide were detected in cocultures of macrophages and OLE1 KO cells than with wild-type or heterozygous strains. Moreover, the disruption of OLE1 significantly reduced fungal virulence in systemic murine infection. Taken together, these results demonstrate that Ole1 regulates the pathobiology of C. parapsilosis via UFA and that the OLE1 pathway is a promising antifungal target.

Original languageEnglish
Pages (from-to)136-145
Number of pages10
JournalInfection and Immunity
Volume79
Issue number1
DOIs
Publication statusPublished - Jan 2011

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Coenzymes
Unsaturated Fatty Acids
Candida
Virulence
Membranes
Yeasts
Macrophages
Essential Fatty Acids
Gene Deletion
Cellular Structures
Growth
Coculture Techniques
Agar
Saccharomyces cerevisiae
Cell Survival
Nitric Oxide
Fungi
Fatty Acids
Salts
Cell Membrane

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Parasitology
  • Infectious Diseases

Cite this

The stearoyl-coenzyme a desaturase 1 is essential for virulence and membrane stress in candida parapsilosis through unsaturated fatty acid production. / NamNguyen, Long; Gácser, A.; Nosanchuk, Joshua D.

In: Infection and Immunity, Vol. 79, No. 1, 01.2011, p. 136-145.

Research output: Contribution to journalArticle

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