The Ste5 scaffold allosterically modulates signaling output of the yeast mating pathway

Roby P. Bhattacharyya, A. Reményi, Matthew C. Good, Caleb J. Bashor, Arnold M. Falick, Wendell A. Lim

Research output: Contribution to journalArticle

199 Citations (Scopus)

Abstract

Scaffold proteins organize signaling proteins into pathways and are often viewed as passive assembly platforms. We found that the Ste5 scaffold has a more active role in the yeast mating pathway: A fragment of Ste5 allosterically activated autophosphorylation of the mitogen-activated protein kinase Fus3. The resulting form of Fus3 is partially active-it is phosphorylated on only one of two key residues in the activation loop. Unexpectedly, at a systems level, autoactivated Fus3 appears to have a negative regulatory role, promoting Ste5 phosphorylation and a decrease in pathway transcriptional output. Thus, scaffolds not only direct basic pathway connectivity but can precisely tune quantitative pathway input-output properties.

Original languageEnglish
Pages (from-to)822-826
Number of pages5
JournalScience
Volume311
Issue number5762
DOIs
Publication statusPublished - Feb 10 2006

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Yeasts
Mitogen-Activated Protein Kinases
Proteins
Phosphorylation

ASJC Scopus subject areas

  • General

Cite this

Bhattacharyya, R. P., Reményi, A., Good, M. C., Bashor, C. J., Falick, A. M., & Lim, W. A. (2006). The Ste5 scaffold allosterically modulates signaling output of the yeast mating pathway. Science, 311(5762), 822-826. https://doi.org/10.1126/science.1120941

The Ste5 scaffold allosterically modulates signaling output of the yeast mating pathway. / Bhattacharyya, Roby P.; Reményi, A.; Good, Matthew C.; Bashor, Caleb J.; Falick, Arnold M.; Lim, Wendell A.

In: Science, Vol. 311, No. 5762, 10.02.2006, p. 822-826.

Research output: Contribution to journalArticle

Bhattacharyya, RP, Reményi, A, Good, MC, Bashor, CJ, Falick, AM & Lim, WA 2006, 'The Ste5 scaffold allosterically modulates signaling output of the yeast mating pathway', Science, vol. 311, no. 5762, pp. 822-826. https://doi.org/10.1126/science.1120941
Bhattacharyya, Roby P. ; Reményi, A. ; Good, Matthew C. ; Bashor, Caleb J. ; Falick, Arnold M. ; Lim, Wendell A. / The Ste5 scaffold allosterically modulates signaling output of the yeast mating pathway. In: Science. 2006 ; Vol. 311, No. 5762. pp. 822-826.
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