The small CAB-like proteins of the cyanobacterium Synechocystis sp. PCC 6803

Their involvement in chlorophyll biogenesis for Photosystem II

Miguel A. Hernandez-Prieto, Tania Tibiletti, Leyla Abasova, Diana Kirilovsky, I. Vass, Christiane Funk

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The five small CAB-like proteins (ScpA-E) of the cyanobacterium Synechocystis sp. PCC 6803 belong to the family of stress-induced light-harvesting-like proteins, but are constitutively expressed in a mutant deficient of Photosystem I (PSI). Using absorption, fluorescence and thermoluminescence measurements this PSI-less strain was compared with a mutant, in which all SCPs were additionally deleted. Depletion of SCPs led to structural rearrangements in Photosystem II (PSII): less photosystems were assembled; and in these, the QB site was modified. Despite the lower amount of PSII, the SCP-deficient cells contained the same amount of phycobilisomes (PBS) as the control. Although the excess PBS were functionally disconnected, their fluorescence was quenched under high irradiance by the activated Orange Carotenoid Protein (OCP). Additionally the amount of OCP, but not of the iron-stress induced protein (isiA), was higher in this SCP-depleted mutant compared with the control. As previously described, the lack of SCPs affects the chlorophyll biosynthesis (Vavilin, D., Brune, D. C., Vermaas, W. (2005) Biochim Biophys Acta 1708, 91-101). We demonstrate that chlorophyll synthesis is required for efficient PSII repair and that it is partly impaired in the absence of SCPs. At the same time, the amount of chlorophyll also seems to influence the expression of ScpC and ScpD.

Original languageEnglish
Pages (from-to)1143-1151
Number of pages9
JournalBBA - Bioenergetics
Volume1807
Issue number9
DOIs
Publication statusPublished - Sep 2011

Fingerprint

Synechocystis
Photosystem II Protein Complex
Cyanobacteria
Chlorophyll
Phycobilisomes
Photosystem I Protein Complex
Carotenoids
Proteins
Fluorescence
Heat-Shock Proteins
Thermoluminescence
Biosynthesis
Iron
Light
Repair
cellulose acetate-butyrate

Keywords

  • Chlorophyll
  • High-light inducible proteins
  • Light-harvesting-like proteins
  • Non-photochemical quenching
  • Photosystem II
  • Synechocystis sp. PCC 6803

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology

Cite this

The small CAB-like proteins of the cyanobacterium Synechocystis sp. PCC 6803 : Their involvement in chlorophyll biogenesis for Photosystem II. / Hernandez-Prieto, Miguel A.; Tibiletti, Tania; Abasova, Leyla; Kirilovsky, Diana; Vass, I.; Funk, Christiane.

In: BBA - Bioenergetics, Vol. 1807, No. 9, 09.2011, p. 1143-1151.

Research output: Contribution to journalArticle

Hernandez-Prieto, Miguel A. ; Tibiletti, Tania ; Abasova, Leyla ; Kirilovsky, Diana ; Vass, I. ; Funk, Christiane. / The small CAB-like proteins of the cyanobacterium Synechocystis sp. PCC 6803 : Their involvement in chlorophyll biogenesis for Photosystem II. In: BBA - Bioenergetics. 2011 ; Vol. 1807, No. 9. pp. 1143-1151.
@article{6702682a7bc94cfbb017167b392c780c,
title = "The small CAB-like proteins of the cyanobacterium Synechocystis sp. PCC 6803: Their involvement in chlorophyll biogenesis for Photosystem II",
abstract = "The five small CAB-like proteins (ScpA-E) of the cyanobacterium Synechocystis sp. PCC 6803 belong to the family of stress-induced light-harvesting-like proteins, but are constitutively expressed in a mutant deficient of Photosystem I (PSI). Using absorption, fluorescence and thermoluminescence measurements this PSI-less strain was compared with a mutant, in which all SCPs were additionally deleted. Depletion of SCPs led to structural rearrangements in Photosystem II (PSII): less photosystems were assembled; and in these, the QB site was modified. Despite the lower amount of PSII, the SCP-deficient cells contained the same amount of phycobilisomes (PBS) as the control. Although the excess PBS were functionally disconnected, their fluorescence was quenched under high irradiance by the activated Orange Carotenoid Protein (OCP). Additionally the amount of OCP, but not of the iron-stress induced protein (isiA), was higher in this SCP-depleted mutant compared with the control. As previously described, the lack of SCPs affects the chlorophyll biosynthesis (Vavilin, D., Brune, D. C., Vermaas, W. (2005) Biochim Biophys Acta 1708, 91-101). We demonstrate that chlorophyll synthesis is required for efficient PSII repair and that it is partly impaired in the absence of SCPs. At the same time, the amount of chlorophyll also seems to influence the expression of ScpC and ScpD.",
keywords = "Chlorophyll, High-light inducible proteins, Light-harvesting-like proteins, Non-photochemical quenching, Photosystem II, Synechocystis sp. PCC 6803",
author = "Hernandez-Prieto, {Miguel A.} and Tania Tibiletti and Leyla Abasova and Diana Kirilovsky and I. Vass and Christiane Funk",
year = "2011",
month = "9",
doi = "10.1016/j.bbabio.2011.05.002",
language = "English",
volume = "1807",
pages = "1143--1151",
journal = "Biochimica et Biophysica Acta - Bioenergetics",
issn = "0005-2728",
publisher = "Elsevier",
number = "9",

}

TY - JOUR

T1 - The small CAB-like proteins of the cyanobacterium Synechocystis sp. PCC 6803

T2 - Their involvement in chlorophyll biogenesis for Photosystem II

AU - Hernandez-Prieto, Miguel A.

AU - Tibiletti, Tania

AU - Abasova, Leyla

AU - Kirilovsky, Diana

AU - Vass, I.

AU - Funk, Christiane

PY - 2011/9

Y1 - 2011/9

N2 - The five small CAB-like proteins (ScpA-E) of the cyanobacterium Synechocystis sp. PCC 6803 belong to the family of stress-induced light-harvesting-like proteins, but are constitutively expressed in a mutant deficient of Photosystem I (PSI). Using absorption, fluorescence and thermoluminescence measurements this PSI-less strain was compared with a mutant, in which all SCPs were additionally deleted. Depletion of SCPs led to structural rearrangements in Photosystem II (PSII): less photosystems were assembled; and in these, the QB site was modified. Despite the lower amount of PSII, the SCP-deficient cells contained the same amount of phycobilisomes (PBS) as the control. Although the excess PBS were functionally disconnected, their fluorescence was quenched under high irradiance by the activated Orange Carotenoid Protein (OCP). Additionally the amount of OCP, but not of the iron-stress induced protein (isiA), was higher in this SCP-depleted mutant compared with the control. As previously described, the lack of SCPs affects the chlorophyll biosynthesis (Vavilin, D., Brune, D. C., Vermaas, W. (2005) Biochim Biophys Acta 1708, 91-101). We demonstrate that chlorophyll synthesis is required for efficient PSII repair and that it is partly impaired in the absence of SCPs. At the same time, the amount of chlorophyll also seems to influence the expression of ScpC and ScpD.

AB - The five small CAB-like proteins (ScpA-E) of the cyanobacterium Synechocystis sp. PCC 6803 belong to the family of stress-induced light-harvesting-like proteins, but are constitutively expressed in a mutant deficient of Photosystem I (PSI). Using absorption, fluorescence and thermoluminescence measurements this PSI-less strain was compared with a mutant, in which all SCPs were additionally deleted. Depletion of SCPs led to structural rearrangements in Photosystem II (PSII): less photosystems were assembled; and in these, the QB site was modified. Despite the lower amount of PSII, the SCP-deficient cells contained the same amount of phycobilisomes (PBS) as the control. Although the excess PBS were functionally disconnected, their fluorescence was quenched under high irradiance by the activated Orange Carotenoid Protein (OCP). Additionally the amount of OCP, but not of the iron-stress induced protein (isiA), was higher in this SCP-depleted mutant compared with the control. As previously described, the lack of SCPs affects the chlorophyll biosynthesis (Vavilin, D., Brune, D. C., Vermaas, W. (2005) Biochim Biophys Acta 1708, 91-101). We demonstrate that chlorophyll synthesis is required for efficient PSII repair and that it is partly impaired in the absence of SCPs. At the same time, the amount of chlorophyll also seems to influence the expression of ScpC and ScpD.

KW - Chlorophyll

KW - High-light inducible proteins

KW - Light-harvesting-like proteins

KW - Non-photochemical quenching

KW - Photosystem II

KW - Synechocystis sp. PCC 6803

UR - http://www.scopus.com/inward/record.url?scp=79960563606&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79960563606&partnerID=8YFLogxK

U2 - 10.1016/j.bbabio.2011.05.002

DO - 10.1016/j.bbabio.2011.05.002

M3 - Article

VL - 1807

SP - 1143

EP - 1151

JO - Biochimica et Biophysica Acta - Bioenergetics

JF - Biochimica et Biophysica Acta - Bioenergetics

SN - 0005-2728

IS - 9

ER -