The slow sarco/endoplasmic reticulum Ca2+-ATPase declines independently of slow myosin in soleus muscle of diabetic rats

Gábor Rácz, András Szabó, A. Vér, E. Zádor

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The sarcoplasmic reticulum Ca2+-ATPase (SERCA) isoforms are normally expressed in coordination with the corresponding myosin heavy chain (MyHC) isoforms in the fibers of skeletal muscle but this coordination is often disrupted in pathological conditions. In the streptozotocin-induced diabetes of rats (stz-rats), the soleus muscle showed peripheral neuropathy and the SERCA2a level decreased in type I (slow-oxidative) fibers compared to the control muscles, whereas the expression of the corresponding slow MyHC1 did not change. No difference was found at the mRNA and protein levels of SERCA and MyHC isoforms in the whole soleus, except that the level of the SERCA2a protein specifically declined in stz-rats compared to the controls. This shows that the coordinated expression of SERCA2a and MyHC1 is disrupted at the SERCA2a protein level in the diabetic soleus. The results are in line with previous observations that regulators of the Ca-homeostasis may adapt faster to type I diabetes than the contractile elements.

Original languageEnglish
Pages (from-to)487-493
Number of pages7
JournalActa Biochimica Polonica
Volume56
Issue number3
Publication statusPublished - 2009

Fingerprint

Sarcoplasmic Reticulum Calcium-Transporting ATPases
Myosins
Medical problems
Muscle
Rats
Protein Isoforms
Skeletal Muscle
Experimental Diabetes Mellitus
Myosin Heavy Chains
Streptozocin
Proteins
Calcium-Transporting ATPases
Fibers
Skeletal Muscle Fibers
Sarcoplasmic Reticulum
Peripheral Nervous System Diseases
Type 1 Diabetes Mellitus
Homeostasis
Muscles
Messenger RNA

Keywords

  • Diabetic rat
  • Muscle fibers
  • Myosin
  • SERCA

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

The slow sarco/endoplasmic reticulum Ca2+-ATPase declines independently of slow myosin in soleus muscle of diabetic rats. / Rácz, Gábor; Szabó, András; Vér, A.; Zádor, E.

In: Acta Biochimica Polonica, Vol. 56, No. 3, 2009, p. 487-493.

Research output: Contribution to journalArticle

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