The single Cdk1-G1 cyclin of Cryptococcus neoformans is not essential for cell cycle progression, but plays important roles in the proper commitment to DNA synthesis and bud emergence in this yeast

Eric V. Virtudazo, Susumu Kawamoto, Misako Ohkusu, Shigeji Aoki, M. Sipiczki, Kanji Takeo

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The cell cycle pattern of the pathogenic basidiomycetous yeast Cryptococcus neoformans differs from that of the ascomycetous budding yeast Saccharomyces cerevisiae. To clarify the cell cycle control mechanisms at the molecular level, homologues of cell cycle control genes in C. neoformans were cloned and analyzed. Here, we report on the cloning and characterization of genes coding for CDK1 cyclin homologues, in particular, the C. neoformans G1 cyclin. We have identified three putative CDK1 cyclin homologues and two putative CDK5 (PHO85) cyclin homologues from the genome. Complementation tests in an S. cerevisiae G1 cyclin triple mutant confirmed that C. neoformans CLN1 is able to complement S. cerevisiae G1 cyclin deficiency, demonstrating that it is a G1 cyclin homologue. Interestingly, cells deleted of the single Cdk1-G1 cyclin were viable, demonstrating that this gene is not essential. However, it exhibited aberrant budding and cell division and a clear delay in the initiation of DNA synthesis as well as an extensive delay in budding. The fact that the mutant managed to traverse the G1 to M phase may be due to the activities of Pho85-related G1 cyclins. Also, that C. neoformans had only a single Cdk1-G1 cyclin highlighted the importance of keeping in order the commitment to the initiation of DNA synthesis first and then that of budding, as discussed.

Original languageEnglish
Pages (from-to)605-618
Number of pages14
JournalFEMS Yeast Research
Volume10
Issue number5
DOIs
Publication statusPublished - 2010

Fingerprint

Cyclin G1
Cryptococcus neoformans
Cell Cycle
Yeasts
DNA
Cyclins
Saccharomyces cerevisiae
Cell Cycle Checkpoints
Cell Division
Genetic Complementation Test
cdc Genes
Saccharomycetales
Genes
Organism Cloning
Genome

Keywords

  • cell cycle
  • Cryptococcus neoformans
  • G1 cyclin

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Microbiology
  • Medicine(all)

Cite this

The single Cdk1-G1 cyclin of Cryptococcus neoformans is not essential for cell cycle progression, but plays important roles in the proper commitment to DNA synthesis and bud emergence in this yeast. / Virtudazo, Eric V.; Kawamoto, Susumu; Ohkusu, Misako; Aoki, Shigeji; Sipiczki, M.; Takeo, Kanji.

In: FEMS Yeast Research, Vol. 10, No. 5, 2010, p. 605-618.

Research output: Contribution to journalArticle

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