The significance of lectin receptors for the evaluation of hormone dependence in breast cancer

P. J. Klein, M. Vierbuchen, J. Fischer, K. D. Schulz, G. Farrar, G. Uhlenbruck

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Abstract

Benign and malignant breast diseases were histochemically investigated with regard to their secretory function. Thereby, the labelled lectin from peanut (peanut agglutinin = PNA) which possesses a high affinity for d-galactosyl-(l-3)-N-acetyl-d-galactosamine, showed a binding pattern partly indicating disturbed secretory cell mechanisms. Further studies with a labelled antibody directed against a milk fat globule membrane glycoprotein, which was isolated by PNA affinity chromatography from human milk, confirmed the PNA receptor as a marker of a milk protein in breast carcinomas. Additionally, two other monoclonal antibodies which were raised against other components of the milk fat globule membrane were used for a more detailed characterization of the functional situation of mammary carcinomas. These immuno- and lectin-histochemical investigations revealed that tumors with a high secretory activity responded in about 80% to endocrine treatment, whereas breast carcinomas that exhibited no secretory function failed to respond to endocrine therapy. Thereby, the hormone dependence of tumor tissue showed an inverse correlation with the proliferative activity, as determined by the mitosis index.

Original languageEnglish
Pages (from-to)839-844
Number of pages6
JournalJournal of Steroid Biochemistry
Volume19
Issue number1 PART 3
DOIs
Publication statusPublished - 1983

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Mitogen Receptors
Peanut Agglutinin
Tumors
Hormones
Breast Neoplasms
Affinity chromatography
Galactosamine
Milk Proteins
Membrane Glycoproteins
Lectins
Breast Diseases
Monoclonal Antibodies
Human Milk
Tissue
Membranes
Affinity Chromatography
Mitosis
Antibodies
Neoplasms
milk fat globule

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

Cite this

The significance of lectin receptors for the evaluation of hormone dependence in breast cancer. / Klein, P. J.; Vierbuchen, M.; Fischer, J.; Schulz, K. D.; Farrar, G.; Uhlenbruck, G.

In: Journal of Steroid Biochemistry, Vol. 19, No. 1 PART 3, 1983, p. 839-844.

Research output: Contribution to journalArticle

Klein, P. J. ; Vierbuchen, M. ; Fischer, J. ; Schulz, K. D. ; Farrar, G. ; Uhlenbruck, G. / The significance of lectin receptors for the evaluation of hormone dependence in breast cancer. In: Journal of Steroid Biochemistry. 1983 ; Vol. 19, No. 1 PART 3. pp. 839-844.
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