The serum and glucocorticoid-regulated kinase 1 in hypoxic renal injury

Krisztina Rusai, Bettina Wagner, Marcel Roos, Christoph Schmaderer, Matthias Strobl, Krishna M. Boini, Almut Grenz, Dietmar Kuhl, Uwe Heemann, Florian Lang, Jens Lutz

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20 Citations (Scopus)

Abstract

The serum-and glucocorticoid-inducible kinase 1 (SGK1) is a serine threonine protein kinase activated through the phosphatidylinositol 3-kinase (PI3-kinase) pathway and counteracting apoptosis. Protein expression and activation of SGK1 are increased in various models of cell stress. The present study explored the role of SGK1 in renal hypoxia/ischemia induced apoptosis. HEK 293 cells were exposed in vitro to hypoxia/reoxygenation (H/R), which increased SGK1 transcript levels, SGK1 protein abundance and SGK1 phosphorylation. H/R injury further enhanced the percentage of apoptotic cells, an effect significantly blunted by prior SGK1 overexpression. In vivo renal ischemia/reperfusion (I/R) injury increased SGK1 transcript levels and SGK1 protein abundance. I/R enhanced apoptosis, an effect significantly more pronounced in gene targeted mice lacking SGK1. In conclusion, SGK1 is up-regulated and counteracts apoptosis following H/R in vitro and ischemia in vivo.

Original languageEnglish
Pages (from-to)577-584
Number of pages8
JournalCellular Physiology and Biochemistry
Volume24
Issue number5-6
DOIs
Publication statusPublished - Jan 1 2009

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Keywords

  • Apoptosis
  • Hypoxia
  • Ischemia/reperfusion
  • Kidney
  • SGK1

ASJC Scopus subject areas

  • Physiology

Cite this

Rusai, K., Wagner, B., Roos, M., Schmaderer, C., Strobl, M., Boini, K. M., Grenz, A., Kuhl, D., Heemann, U., Lang, F., & Lutz, J. (2009). The serum and glucocorticoid-regulated kinase 1 in hypoxic renal injury. Cellular Physiology and Biochemistry, 24(5-6), 577-584. https://doi.org/10.1159/000257527