The separation of the granulocytes from different rat strains A comparative study

S. Szücs, C. Varga, I. Ember, P. Kertai

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Different Percoll density gradients were used to purify granulocytes from Long-Evans, Fischer, Sprague-Dawley, and Fischer/Long Evans hybrid rats. Three different discontinuous gradient types were developed which permitted the separation of polymorphonuclear leukocytes (PMNLs) from the peripheral blood of the different rat strains. Our purification techniques were compared to each other in terms of purity and yield. Purities of 97.7 ± 0.6%, 97.0 ± 1.1%, 96.4 ± 1.2% and 96.7% ± 1.1% were achieved for the granulocyte fractions of LE, F344, SD and FL/F1 rats, respectively. The superoxide production of the isolated cells was also investigated and it was established that the granulocytes could be activated by phorbol-12-myristate-13-acetate (PMA).

Original languageEnglish
Pages (from-to)245-251
Number of pages7
JournalJournal of Immunological Methods
Volume167
Issue number1-2
DOIs
Publication statusPublished - Jan 3 1994

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Granulocytes
Long Evans Rats
Superoxides
Acetates
Neutrophils

Keywords

  • Cell separation
  • Granulocyte
  • Percoll gradient
  • Rat strain
  • Superoxide production

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

Cite this

The separation of the granulocytes from different rat strains A comparative study. / Szücs, S.; Varga, C.; Ember, I.; Kertai, P.

In: Journal of Immunological Methods, Vol. 167, No. 1-2, 03.01.1994, p. 245-251.

Research output: Contribution to journalArticle

Szücs, S. ; Varga, C. ; Ember, I. ; Kertai, P. / The separation of the granulocytes from different rat strains A comparative study. In: Journal of Immunological Methods. 1994 ; Vol. 167, No. 1-2. pp. 245-251.
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