The sensitivity of Photosystem II to damage by UV-B radiation depends on the oxidation state of the water-splitting complex

András Szilárd, L. Sass, Z. Deák, I. Vass

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The water-oxidizing complex of Photosystem II is an important target of ultraviolet-B (280-320 nm) radiation, but the mechanistic background of the UV-B induced damage is not well understood. Here we studied the UV-B sensitivity of Photosystem II in different oxidation states, called S-states of the water-oxidizing complex. Photosystem II centers of isolated spinach thylakoids were synchronized to different distributions of the S0, S1, S2 and S3 states by using packages of visible light flashes and were exposed to UV-B flashes from an excimer laser (λ = 308 nm). The loss of oxygen evolving activity showed that the extent of UV-B damage is S-state-dependent. Analysis of the data obtained from different synchronizing flash protocols indicated that the UV-sensitivity of Photosystem II is significantly higher in the S3 and S2 states than in the S1 and S0 states. The data are discussed in terms of a model where UV-B-induced inhibition of water oxidation is caused either by direct absorption within the catalytic manganese cluster or by damaging intermediates of the water oxidation process.

Original languageEnglish
Pages (from-to)876-882
Number of pages7
JournalBBA - Bioenergetics
Volume1767
Issue number6
DOIs
Publication statusPublished - Jun 2007

Fingerprint

Photosystem II Protein Complex
Radiation
Oxidation
Water
Background Radiation
Excimer Lasers
Thylakoids
Spinacia oleracea
Excimer lasers
Manganese
Oxygen
Light

Keywords

  • Oxygen evolution
  • Photosystem II
  • S-states
  • UV-damage

ASJC Scopus subject areas

  • Biophysics

Cite this

The sensitivity of Photosystem II to damage by UV-B radiation depends on the oxidation state of the water-splitting complex. / Szilárd, András; Sass, L.; Deák, Z.; Vass, I.

In: BBA - Bioenergetics, Vol. 1767, No. 6, 06.2007, p. 876-882.

Research output: Contribution to journalArticle

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